qPCR in gastrointestinal stromal tumors: Evaluation of reference genes and expression analysis of KIT and the alternative receptor tyrosine kinases FLT3, CSF1-R, PDGFRB, MET and AXL

Table 1 Selected candidate reference genes for gene expression analysis.

Abbreviation	Gene name	Cellular function
18S rRNA	18S ribosomal RNA	ribosome subunit	X03205.1
ACTB	ß-actin	cytoskeleton	NM_001101.3
B2M	ß-2-microglobulin	major histocompatibility complex	NM_004048.2
GAPDH	glyceraldehyde-3-phosphate dehydrogenase	glycolysis enzyme	NM_002046.3
GUSB	ß-glucuronidase	glycosaminoglycan degradation	NM_000181.2
HMBS	hydroxymethylbilane synthase	heme production	NM_000190.3
HPRT1	hypoxanthine ribosyltransferase	metabolic salvage of purines	NM_000194.2
IPO8	importin 8	intracellular protein transport	NM_006390.2
PGK1	phosphoglycerate kinase 1	glycolysis enzyme	NM_000291
POLR2A	DNA-dependent RNA polymerase II	transcription	NM_000937.3
PPIA	peptidyl-prolyl isomerase A	protein folding	NM_021130
RPLPO	ribosomal phosphoprotein, large, P0	ribosome	NM_001002
TBP	TATA-box-binding protein	transcription factor	NM_003194
TFRC	transferrin receptor	cellular iron uptake	NM_021009.4
UBC	ubiquitin C	posttranslational modification	NM_003406.3
YWHAZ	tyrosine 3-/tryptophan 5-Monooxygenase-activation protein, zeta isoform	signal transduction	NM_003404

ISSN: 1471-2199