Figure 3

Distribution overview of expression levels (Ct) of the different genes. Boxplot representation of raw Ct values obtained from amplification curves. Lower and upper boundaries of the box indicate the 25th and the 75th percentile, respectively, the thin line within the box marks the median, and the whiskers (error bars) below and above the box indicate the 10th and 90th percentiles. Mean (thick line) and outliers (*). Complete RNA sample set from the study (n = 32, grey), sample set "A" (n = 9, yellow; see Figure 2, Glucose + Galactose) and sample set "K" (n = 11, green; see Figure 2, WT + tps1Δ). As stated in methods, the 25 μL reaction mixes contained 5 μL of cDNA preparation diluted 10 times, except for RDN18 where cDNA was diluted 50 times. For a easy and preliminary estimation of the relative expression of a gene between two samples, a difference of 3.33 Ct with 100% PCR efficiency represents 10-fold over-expression or repression between two conditions (N₂/N₁ = (1+Eff)^(Ct₁-Ct₂)); With PCR efficiency correction, the same Ct difference with only 90% efficiency signifies a 8.5-fold variation of transcripts between the two samples.