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Figure 6 | BMC Molecular Biology

Figure 6

From: Interaction of ZEB and Histone Deacetylase with the PLDLS-binding cleft region of monomeric C-terminal Binding Protein 2

Figure 6

HDAC-dependent transcriptional repression by Gal4-RR-CtBP2. A, Transcriptional repression by Gal4-ZEB101. Gal4 fusion constructs were co-transfected into MCF7 cells with pG5-Luc and phRL-tk. Dual luciferase assay was performed as in Fig. 3A. DBD: Gal4 DNA binding domain. B, Failure of RR-CtBP2 to repress transcription through Gal4-ZEB101. CtBP1/2 double knockout cells were co-transfected with pG5-Luc, phRL-tk, Gal4-ZEB101 and CtBP2 or RR-CtBP2. Dual luciferase assay was performed as in A. C, HDAC-dependent transcriptional repression by Gal4-CtBP2 and Gal4-RR-CtBP2. MEF90 cells were co-transfected with pG5-Luc, phRL-tk, and Gal4-CtBP2 or Gal4-RR-CtBP2. HDAC inhibitor TSA was added at 0.2 ng/ml during transfection. Dual luciferase assay was carried out as in A.

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BMC Molecular Biology

ISSN: 1471-2199

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