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Figure 4 | BMC Molecular Biology

Figure 4

From: Interaction of ZEB and Histone Deacetylase with the PLDLS-binding cleft region of monomeric C-terminal Binding Protein 2

Figure 4

Roles of PLDLS-like motifs of ZEB in interaction with CtBP2 and RR-CtBP2. A, Diagram of constructs. Numbers denote the amino acid residue number in the full-length ZEB1. B, Interaction of CtBP2 and RR-CtBP2 with GST-ZEB101 and GST-E1A. GST-ZEB101, GST-E1A, and GST-E1A-C* (with a DL→AS mutation in PLDLS motif) were charged to glutathione beads and then incubated with purified H6-CtBP2 or H6-RR-CtBP2. Bound CtBP2 was examined by western blot with the CtBP2 antibody and GST-fusion proteins visualized by Coomassie blue. Input (lanes 1 and 2) represents 5% of input proteins. C, Interaction of CtBP2 and RR-CtBP2 with mutants of GST-ZEB101. Conditions were the same as in B, except that Input (lane 1) represent 10% of input proteins. Bottom panel: Coomassie blue-stained GST-fusion proteins for the binding assays involving H6-CtBP2 (top panel). GST-fusion proteins for the binding of H6-RR-CtBP2 were the same (not shown).

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BMC Molecular Biology

ISSN: 1471-2199

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