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Figure 1 | BMC Molecular Biology

Figure 1

From: Interaction of ZEB and Histone Deacetylase with the PLDLS-binding cleft region of monomeric C-terminal Binding Protein 2

Figure 1

Analysis of dimerization of CtBP2 and its mutants. A, Dimerization of endogenous CtBP proteins. HeLa cells were treated with DSS and Western blots performed with CtBP1 and CtBP2 antibodies (Pharmingen). B, Dimerization of transiently expressed CtBP proteins. Various Flag-HA-tagged CtBP constructs were transfected into HeLa cells. One day after transfection, cells were treated with DSS and cell lysates were examined by Western blot analysis using the Flag antibody. C, Dimerization defect of RR-CtBP2 and GG-CtBP2. CtBP2-HBH, RR-CtBP2-HBH, or GG-CtBP2-HBH was co-transfected with the Flag-HA-tagged version in indicated combinations. Biotin (5 μM) was added to cells after addition of DNA precipitates. After immunoprecipitation with the streptavidin (SA) agarose beads, bound proteins were analyzed by western blot with a CtBP2 antibody to detect FH-CtBP2, or with the streptavidin-HRP conjugate to detect CtBP2-HBH proteins.

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BMC Molecular Biology

ISSN: 1471-2199

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