Figure 5From: Functional mapping of the fission yeast DNA polymerase δ B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesisDominant negative Cdc1 mutants. cdc1-P13 cells carrying plasmids pREP3X, pREP3XH6BN-Cdc1, pREP3XH6BN-Cdc1-J7 or pREP3XH6BN-Cdc1-A10 were grown up in EMM medium at 28°C before being plated onto EMM plates with (+T, right hand panels) or without 5 μg/ml thiamine (-T, left hand panels) and incubated for 4 days at 28°C.Back to article page