Table 3 qPCR primer sequences, and amplification parameters.
From: Selection of reference genes for expression studies with fish myogenic cell cultures
Gene | Primer sequence (5'-3') | Amplicon size (bp) | Tm (°C) | E (%) plasmid | R2 plasmid | E (%) cDNA | R2 cDNA |
|---|---|---|---|---|---|---|---|
EF1α | f: GAATCGGCTATGCCTGGTGAC r: GGATGATGACCTGAGCGGTG | 141 | 86.0 | 96.0 | 0.998 | 99.5 | 0.999 |
RNApolII | f: CCAATACATGACCAAATATGAAAGG r: ATGATGATGGGGATCTTCCTGC | 157 | 84.8 | 95.3 | 0.996 | 98.5 | 0.999 |
18SrRNA | f: TCGGCGTCCAACTTCTTA r: GCAATCCCCAATCCCTATC | 189 | 86.5 | 95.6 | 0.995 | 94.5 | 0.999 |
Ppia | f: CATCCCAGGTTTCATGTGC r: CCGTTCAGCCAGTCAGTGTT | 203 | 85.9 | 96.4 | 0.998 | 96.5 | 0.999 |
Pgk1 | f: CTCGGTGATGGGGCTTAGG r: TCATTGGTGGAGGCGACA | 160 | 87.0 | 98.1 | 0.999 | 99.5 | 0.999 |
Actb | f: TGACCCAGATCATGTTTGAGACC r: CTCGTAGATGGGTACTGTGTGGG | 146 | 83.8 | 93.2 | 0.997 | 100 | 0.999 |
Hprt1 | f: CCGCCTCAAGAGCTACTGTAAT r: GTCTGGAACCTCAAACCCTATG | 255 | 81.8 | 92.1 | 0.996 | 90.0 | 0.997 |
Sdha | f: CATGTTACCAAGGGCTGCAT r: GTGTCAGATGATATCTCAACCCAG | 207 | 85.8 | 99.0 | 0.997 | 95.5 | 0.998 |
Des | f: GTCCATCTGGATCTGCACCT r: GGCTGCTTTCAGAGCTGATG | 169 | 82.8 | 99.5 | 0.998 | 99.0 | 0.996 |