Skip to main content
Figure 1 | BMC Molecular Biology

Figure 1

From: Targeted suppression of heme oxygenase-1 by small interference RNAs inhibits the production of bilirubin in neonatal rat with hyperbilirubinemia

Figure 1

Detection of siRNA transfection efficiency. Fifty nmol/L FAM labeled NC-siRNA was transfected into the cells for 6 hours. The nuclei were stained with DAPI. Confocal microscopy (×60) and flow cytometry were employed to detect the transfection efficiency (upper figure A, B and C), siRNA entered the cells, and primarily distributed in the cytoplasm. The lower figure was the result from flow cytometric analysis, D contained untransfected cells, E contained FAM-siRNA transfected cells. The results were analyzed with Win MDI29 software, which indicated that the siRNA transfection efficiency in BRL cells was approximately 90%.

Back to article page

BMC Molecular Biology

ISSN: 1471-2199

Contact us