Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies

Table 1 Classification of Balanus amphitrite ESTs

Vector clipped ESTs longer than 50 nucleotides and with an E-value > e-5 were functionally annotated whenever possible. Blast hits with E-values < e-5 were included in the unassigned proteins. Unknown ESTs in our dataset are those with no match in sequence databases. These ESTs were considered to be of genomic DNA origin as it is unlikely that the highly conserved mitochondrial gene would not result in a Blast hit.

ISSN: 1471-2199