Figure 4

The Os03g29760 transcript is a target gene of miR-169. The mRNA levels of the predicted target genes of miR-169 were quantified by RT-PCR (A) and quantitative real-time PCR (B). The mRNA levels of the predicted target genes were quantified by quantitative real-time RT-PCR and normalized to the level of β-tubulin. Error bars represent the standard deviations of three PCR replicates of a single reverse transcription reaction. The normalized mRNA levels in untreated sample were arbitrarily set to 1. (C) The amplified product of the 5' RACE on the Os03g29760 transcript by the primer150 and primer 180. (D) The 5'end of the Os03g29760 determined by 5' RACE.