Table 6 Integration of Hermes episomes into target DNA in vitro.

Site^a	N^b	Orientation^c	Target^d	Site^a	N^b	Orientation^c	Target^d
		n = 0 ⁵				n = 1 ⁵
2	1	-	TTATAAAA	209	1	-	TTAAGTGG
94	1	-	ATTGAGAT	389	1	-	GTTTATAT
343	1	-	GGATATAT	2358	1	-	ATTCAGAG
423	1	-	GTTGGGAT	232	1	+	CCCTCAAC
1665	1	-	TTACCAAT	249	1	+	GTTTTGAT
2394	2	-	GTATGTAC	339	2	+	TTTGGGAT
94	1	+	ATTGAGAT	431	1	+	AGACGTAA
232	1	+	CCCTCAAC	438	1	+	ATATGCGT
313	2	+	TATGAGTA	624	1	+	GTCGTAAT
339	1	+	TTTGGGAT	708	1	+	GTAAAGAT
370	1	+	TAAAGCAC	731	1	+	GTTATGTC
423	1	+	GTTGGGAT	991	1	+	TTTAAAAG
505	1	+	TTATCGAC	1001	1	+	AATGAAAA
603	1	+	TTAAGAAA	2321	1	+	ATTGGAAT
638	1	+	CCAGAAAC
1904	1	+	ATAGCAAC
2154	1	+	GTATGCAC
2303	1	+	GTTCCGAC
		n = 4 ⁵				n = 5 ⁵
94	2	-	ATTGAGAT	94	7	-	ATTGAGAT
135	1	-	ATGCAAAT	156	1	-	TGGAAAAT
389	1	-	GTTTATAT	948	1	-	TTGGAGAT
144	1	+	ATTCAAAT	2433	1	-	GATAATAC
274	1	+	GAATTTGA	123	1	+	ATAAAAAC
318	1	+	GTACAGAG	154	1	+	TTTGGAAA
676	1	+	ATTTATTA	227	1	+	ACCACCCC
804	1	+	TTGATGAT	318	1	+	GTACAGAG
925	1	+	ATATAAAT	350	1	+	TTACAAAC
990	1	+	CTTTAAAA	425	1	+	TGGGATAG
2076	1	+	GAGACATT	612	1	+	CTATTTTT
2428	1	+	TACCAGAT	666	1	+	ACAGTGAT
				710	1	+	AAAGATAA
		n = 17 ⁵		755	1	+	GTAGAAAT
927	1	+	ATAAATAC	777	1	+	ATATGGTT
708	1	+	GTAAAGAT	1029	1	+	ATTTTTGG
				1855	1	+	GAAGTGAA
		n = 37 ⁵		2091	1	+	CCCATCAA
753	1	+	GTGTAGAA	2115	1	+	AGCAACAA
				2303	1	+	GTTCCGAC
		n = 69 ⁵		2322	1	+	TTGGAATA
316	1	-	GAGTACAG	2394	1	+	GTATGTAC
				2429	1	+	ACCAGATA
		n = 120 ⁵
736	3	+	GTCTGAAC
983	1	+	AAATCGAC

^1"Site" of integration; nucleotide number of the first base of the target sequence.
²Number of occurrences observed.
³"+" refers to <left-Hermes-right> relative to target sequence; "-" is <right-left>
⁴"Target" is the first 8 bp following the right ITR and is written 5'-3'
⁵ number of nucleotides separating the left and right ITRs of Hermes.

ISSN: 1471-2199