Figure 1

Structure of the autonomous Hermes elements used in this study. Hermes terminal sequences are shown as thick black arrows with associated nucleotide numbers in parenthesis. Primers used are thin arrows with their corresponding name. The position of relevant restriction endonuclease sites are shown using conventional abbreviations (BamHI, EcoRI, MspI). Hermes ORF - the complete Hermes transposase open reading frame. hsp70 5'-promoter from the hsp70 gene of D. melanogaster. hsp70 3' - 3' untranslated region of the hsp70 gene of D. melanogaster. Actin5C - promoter from the Actin5C gene of D. melanogaster. EGFP- the complete coding region for Enhanced Green Fluorescent Protein. SV40 3'- 3' untranslated region of Simian Virus 40. Ori - ColE 1 origin of replication. Kanamycin - kanamycin resistance gene.