Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions

Table 1 Descriptive statistics and expression level analyses of the tested candidate reference genes based on their crossing point (CP) values

Factor	actin	adh	14-3-3	ccs	gapdh	poly	rpl7	cys
N	21	21	21	21	21	21	21	21
GM [CP]	32.72	29.7	29.63	26.67	22.86	28.46	30.76	26.67
AM [CP]	32.74	29.71	29.64	26.67	22.88	28.49	30.77	26.7
Min [CP]	31.4	28.64	28.94	25.92	21.74	26.79	30.13	25.24
Max [CP]	34.35	31.12	31.21	27.37	23.77	30.07	31.93	28.44
SD [± CP]	1.23	0.99	0.92	0.58	0.94	1.39	0.69	1.31
CV [%CP]	3.75	3.35	3.09	2.19	4.12	4.89	2.24	4.89
Min [x-fold]	0.41	0.43	0.66	0.53	0.54	0.18	0.68	0.41
Max [x-fold]	3.04	3.1	2.62	1.83	1.64	5.22	2.02	3.04
SD [± x-fold]	1.07	1.08	0.82	0.52	0.52	2.09	0.53	1.08

Abbreviations: N: number of samples; CP: crossing-point; GM [CP]: geometric CP mean; AM [CP]: arithmetic CP mean; Min [CP] and Max [CP]: CP threshold values; SD [± CP]: CP standard deviation; CV [%CP]: variance coefficient expressed as percentage of CP level; Min [x-fold] and Max [x-fold]: threshold expression levels expressed as absolute x-fold over- or under-regulation coefficient; SD [± x-fold]: standard deviation of absolute regulation coefficient.

ISSN: 1471-2199