Figure 3

Evaluation of the expression of selected reference genes during fungus infection. The expression of selected reference genes (gapdh, rpl7 and 14-3-3) and of a commonly used coffee normalization gene (ubiquitin) was monitored in leaves of C. arabica var. Mundo Novo inoculated with Hemileia vastatrix. The crossing point (CP) difference (ΔCP = CP_{inoculated leaf} - CP_{non-inoculated leaf}) was calculated for each time-point (8, 12 and 24 h after challenge by the rust fungus) to investigate the expression levels of each reference gene. The standard error of the triplicates for each time-point is indicated by horizontal bars.