The promoter regions of the Myb-regulated Adora2B and Mcm4 genes co-localize with origins of DNA replication

Background The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the transformation of myelomonocytic cells by avian myeloblastosis virus (AMV). v-Myb is thought to exert its biological effects by deregulating the expression of specific target genes. We have recently demonstrated that the chicken Gas41 gene, whose promoter co-localizes with an origin of DNA replication, is a bona fide Myb target gene. Because of this finding we have asked whether other Myb-regulated genes are also associated with DNA replication origins. Results We show that the promoter region of the chicken adenosine receptor 2B gene (Adora2B), a known Myb-target gene, acts as a DNA replication origin. Furthermore, we have examined known replication origins for the presence of Myb binding sites. We found that the intergenic region between the genes for the minichromosome maintenance 4 protein (Mcm4) and the catalytic subunit of DNA-dependent protein kinase (Prkdc), whose human counterpart has been identified as a replication origin, contains a number of Myb binding sites. Our data show that this region also acts as an origin of replication in chicken cells. Interestingly, we found that the chicken Mcm4 gene is also Myb-regulated. Conclusion Our work identifies the chicken Mcm4 gene as a novel Myb target gene and presents evidence for the co-localization of two novel origins of DNA replication with Myb-regulated genes. Our work raises the possibility that a fraction of Myb target gene promoters is associated with DNA replication origins.


Background
The oncogene v-myb of avian myeloblastosis virus (AMV) encodes a transcription factor which is responsible for the ability of AMV to transform cells of the myelomonocytic lineage [1,2]. v-myb is derived from the chicken c-myb gene, which plays a crucial role in the development of the hematopoietic system. c-myb is expressed in immature cells of all hematopoietic lineages and is silenced during the terminal differentiation of these cells. It is thought that c-myb is part of a genetic switch that allows hematopoietic progenitor cells to choose between alternative fates, such as proliferation, differentiation or apoptosis [2,3].
Most of the work aimed at understanding how Myb activates the expression of its target genes has focused on the promoter regions of these genes and has provided a relatively simple picture of how Myb affects gene expression. In many cases, Myb binding sites have been identified in the promoter regions of these genes, suggesting that Myb acts primarily on the promoters of its targets. Recent indepth analyses of some Myb target genes, however, have suggested a more complex picture in which the activation of certain target genes is mediated by cooperation of additional Myb-responsive enhancer elements with the target gene promoters. For example, the expression of the chicken mim-1 gene is controlled by interactions of Myb both with the promoter as well as with a powerful cellspecific enhancer located upstream of the gene [16]. Similarly, in case of the chicken C/EBPβ gene Myb targets the promoter as well as an enhancer located downstream of the gene [17].
Recently, another regulatory DNA element has been mapped close to a Myb-regulated gene. We have shown that chicken Gas41, whose promoter region has been known for some time to act as an origin of DNA replication [18,19], is a direct Myb target gene [20]. Other work has shown that the Myb homolog of Drosophila melanogaster is involved in the control of the replication origin responsible for chorion gene amplification [21]. These observations raised the question of whether DNA replication origins are also associated with other Myb target genes. Here, we demonstrate that the promoter region of the Myb-regulated chicken adenosine receptor 2B gene acts as an origin of DNA replication. Furthermore, we identify the chicken Mcm4 gene as a novel Myb target gene and demonstrate that the Mcm4 promoter is associated with a replication origin. Besides identifying two new DNA replication origins in chicken cells and a novel Mybregulated gene our work suggests that a significant fraction of Myb-regulated genes might be associated with DNA replication origins.

The chicken Mcm4 gene is a Myb target gene
The observation that the promoter of the Myb-regulated the chicken Gas41 gene [20] is associated with an origin of DNA-replication [18,19] prompted us to explore the possibility that other Myb target regions also co-localize with DNA replication origins. Ladenburger et al [22] have previously identified a replication origin located between the human Mcm4 and Prkdc genes. Both genes are separated from each other by an intergenic region of only 0.8 kb and are transcribed in opposite directions. This arrangement is conserved among humans, mice and chickens [23,24]. We noted that the corresponding chicken sequence contains a number of potential Myb binding motifs (Fig. 1). One of the sites (MBS-6) matched exactly to the consensus Myb binding motif PyAAC(G/T)G [4], while the other sites had one (MBS-4, MBS-1) or two (MBS-2, MBS-3 and MBS-5) mismatches. The human Mcm4-Prkdc intergenic region also contains 8 potential Myb binding motifs although their exact positions are not conserved between the human and the chicken sequence. To demonstrate that Myb recognizes at least some of the motifs found in the chicken sequence those sites (MBS-1, MBS-4 and MBS-6) that showed the best fit to the consensus binding motif were analyzed by electrophoretic mobility shift assays (EMSA) using recombinant v-Myb protein expressed in bacteria. As illustrated in Fig. 2A, retarded protein-DNA complexes were observed when oligonucleotides containing MBS-1, MBS-4 and MBS-6 were used, demonstrating that v-Myb is able to recognize at least three of the potential binding sites in vitro. To confirm the specificity of binding we performed a competition experiment in which binding of v-Myb to a radiolabeled oligonucleotide containing the high affinity Myb binding site A from the mim-1 promoter [5] was competed by excess of unlabeled oligonucleotides containing MBS-1, MBS-4 and MBS-6 ( Fig.  2B). Binding was competed efficiently by the mim-1 A site itself but not by a control oligonucleotide containing a C/ EBP binding site. Oligonucleotides containing MBS-4 and MBS-6 also competed, although less efficiently that the mim-1 A oligonucleotide. MBS-1 appeared not to compete specifically with the mim-1 A site. Taken together, this experiment confirmed that v-Myb binds specifically to binding sites 4 and 6 while binding to MBS-1 was quite weak.
To investigate whether v-Myb is bound to the Mcm4 promoter region in vivo we performed chromatin immunoprecipitation (ChIP) experiments using the v-Myb transformed myeloblast line BM2. The cells were formaldehyde-fixed and DNA-protein complexes obtained after sonication were immunoprecipitated with two different antibodies against Myb. As a control, parallel incubations with non-immune serum or with unrelated antibodies were performed. After reverse-crosslinking and purification of the immunoprecipitated DNA the samples were analyzed by quantitative real-time PCR using the primers that bind to the Mcm4-Prkdc intergenic region or control primers. The result of the ChIP experiment is illustrated in Fig. 3. We observed substantial enrichment of the Mcm4-Prkdc intergenic region in samples precipitated with the Myb-specific antibodies, whereas control immunoprecipitations with non-immune sera or unrelated antibodies showed no specific enrichment. PCR-reactions with control primers that were specific for sequences located 13 kilobasepairs upstream of Mcm4 did not show significant enrichment in any of the immunoprecipitations. Taken together, the ChIP experiment clearly showed that v-Myb was bound to the Mcm4-Prkdc intergenic region in vivo.
Next, we investigated whether v-Myb also affected the expression of the Mcm4 or Prkdc genes. To address this issue we made use of a cell line, designated 10.4, which stably expresses a v-Myb/estrogen receptor fusion protein [25]. Upon β-estradiol treatment of 10.4 cells the v-Myb/ ER fusion protein is activated and upregulates the expression of a number of Myb target genes. 10.4 cells were cultivated for 24 hours in the presence or absence of 2 µM β-estradiol. Polyadenylated RNA from these cells was then isolated and analyzed by Northern blotting with probes specific for Mcm4 or Prkdc mRNA. As a control, a probe hybridizing to the ribosomal protein S17 mRNA was used. As shown in Fig. 4, the Mcm4 mRNA level was elevated in the estrogen-treated 10.4 cells when compared to untreated cells, suggesting that chicken Mcm4 is indeed a Myb target gene. By contrast, Prkdc was not regulated by v-Myb. 10.4 cells do not express endogenous estrogen receptor [25], suggesting that Mcm4 is not simply an estrogen-regulated gene.

An origin of DNA replication is located upstream of the chicken Mcm4 gene To investigate whether the intergenic region between
Mcm4 and Prkdc also acts as a DNA replication origin in chicken cells we performed nascent strand abundance assays as described by Giacca et al. [26]. The nascent strand abundance assay is a powerful and widely used method to identify and map DNA replication origins. The assay is based on the isolation and quantification of single stranded DNA 1-2 kb in length (so-called nascent DNA) which is primarily derived from replication origin regions. The method can be performed with asynchronously growing cells and does not depend on the availability of antisera against origin binding proteins (such as Orc-or MCMproteins). Briefly, nascent DNA 1-2 kb in length was isolated from different chicken cells by sucrose gradient sedimentation. Sets of primers mapping to the intergenic region as well as to upstream and downstream sequences were used to determine the abundance of the corresponding sequences in nascent DNA using quantitative realtime PCR. BM2 cells are myeloid v-myb transformed cells that express high levels of the v-Myb protein. HD11 cells are myeloid cells that do not express v-Myb or c-Myb. DT40 is a line of B-lymphoid cells that express c-Myb. Finally, we used a subclone of the DT40 line in which the c-myb gene had been disrupted by homologous recombination [27]. As illustrated in Fig. 5 the Mcm4-Prkdc intergenic region was enriched in nascent DNA in each of these cell lines. This demonstrated that this region acts as a DNA replication origin also in chicken cells. We did not observe significant differences in the abundance of the origin region between myeloid cells expressing (BM2) or not expressing (HD11) v-Myb. Although the abundance of the origin region was higher in DT40 cells compared to myeloid cells, there was no significant difference between wild-type and c-Myb deficient DT40 cells. Thus, although Myb binds to the intergenic region and stimulates Mcm4 expression, it does not affect the activity of the replication origin.

Identification of an origin of DNA-replication in the promoter region of the chicken adenosine receptor 2B gene
We also examined the promoters of two known Myb-regulated genes, the mim-1 and adenosine 2B receptor genes, for replication origin activity. Both genes are direct Myb targets and their promoters have been analyzed in detail  N R S (a ) N R S (b ) a n ti F la g a n ti M y b (a ) a n ti M y b (b ) N R S (a ) N R S (b ) a n ti F la g [5,14,[28][29][30]. Nascent DNA from different cell lines was analyzed using sets of primers spanning a region from -5 kb to +5 kb around the promoter in case of the mim-1 gene and from -3.2 kb to +4.8 kb around the promoter of the A2B receptor gene. We did not observe enrichment of mim-1 promoter sequences in preparations of nascent DNA, indicating the mim-1 promoter does not coincide with a DNA replication origin (data not shown). By contrast, the promoter region of the A2B receptor gene was enriched in nascent DNA, suggesting that an origin of DNA-replication is located at the 5' end of the A2B receptor gene (Fig. 6). As in the case of the Mcm4 replication origin we did not observe significant differences in origin activity of the promoter region of the A2B receptor gene between the different cell lines.

Discussion
The identification of the chicken Gas41 gene, whose promoter region functions as a DNA replication origin, as a bona fide Myb target gene [20] has raised the question whether the co-localization of a Myb-regulated promoter with a replication origin is just a rare coincidence or a more common phenomenon? To address this question we used two different strategies: i.) We examined the promoter regions of known Myb-regulated genes for replication origin activity using the nascent strand abundance assay. ii.) We investigated the activity of v-Myb at the Mcm4-Prkdc intergenic region, which harbors several potential Myb binding sites and is associated with a replication origin in human cells [22].
Analysis of the adenosine receptor 2B gene, a known Myb target gene [14,30], showed that the sequences around the 5' end of the gene were enriched in preparations of nascent DNA. This observation strongly suggested that the promoter of the A2B receptor co-localizes with a DNA replication origin. A further case of such co-localization was revealed by the analysis of the Mcm4-Prkdc intergenic region. Our data showed that v-Myb binds to this region both in vitro and in vivo and stimulates transcription of the chicken Mcm4 gene. Furthermore, we demonstrated that the Mcm4-Prkdc intergenic region is a replication origin also in chicken cells. Thus, chicken Mcm4 is a novel v-Myb target gene whose promoter region is also associated with a DNA replication origin. As these examples show, Myb-regulated genes and replication origins co-localize in some cases. However, it is also evident from the limited number of genes that have been analyzed so far that not every Myb target gene promoter is associated with a replication origin. For example, the promoter region of the mim-1 gene does not possess replication origin activity.
In the light of the co-localization of replication origins with several promoters containing clusters of Myb binding sites we wondered how frequently such clusters occur in the chicken genome. We screened approximately three million basepairs of randomly selected chicken genomic sequences for the presence of clusters of Myb binding motifs (AACG or AACTG) that are similar to those found at the MCM4, Adora2b and Gas41 replication origins. While on the average one such motif is present per 200 base pairs we found that clusters of at least six of such sites within stretches of 250 base pairs occur only once per approximately 25 kb. Although the number of such binding motif clusters is higher than the estimated number of replication origins in the chicken genome (one replication origin per approximately 100 kb, as extrapolated from mammalian cells [31,32]), it appears unlikely that the concurrence of clusters of Myb binding motifs and DNA replication origins reported here is purely accidental. It will therefore be interesting to examine such clusters of Myb binding motifs for their potential role as replication origins.

Replication origin activity of the Mcm4-Prkdc intergenic region in chicken cells
The functional consequences, if any, of such co-localization are unclear at present. We have not observed that v-Myb or c-Myb influence the activity of the replication origins identified here. v-Myb or c-Myb, therefore, appear not to be involved in the control of origin activity. Recent work on the Myb homolog (Dm-Myb) of Drosophila melanogaster has provided evidence that Dm-Myb performs a non-transcriptional role in the control of the replication origin responsible for chorion gene amplification [21]. Furthermore, it has been shown that some of the phenotypic consequences of mutation of Dm-Myb can be compensated by B-Myb, but not by c-Myb or A-Myb [33]. It is therefore conceivable that B-Myb plays a role at the replication origins identified here. Another interesting possibility is that rather than being regulated by Myb the presence of a replication origin might affect the activation of the associated gene by Myb. For example, compacted chromatin structures will be opened during replication thereby providing transcription factors access to the DNA. It is therefore possible that the association with a replication origin imposes an additional level of control onto the adjacent gene, which would be expected to be cell cycle dependent. It is presently not known whether the Mcm4 and Adora2B genes are expressed in a cell cycle dependent manner. However, in this respect it is interesting to note that the phenotypic changes of myeloid cells transformed by a temperature-sensitive version of v-Myb that accompany activation of v-Myb by down-shifting the cells from the non-permissive to the permissive temperature were not observed when DNA replication was inhibited [34]. This observation is consistent with the idea that the activation of at least some Myb-regulated genes depends on DNA replication. Clearly, further work is necessary until we can discern which of these possibilities, if any, is right.
Independent of a possible role of DNA replication origins in Myb-inducible gene expression our work is also of interest because we have identified two novel replication origins in chicken cells. So far, only very few replication origins are known in chicken cells, such as the origins located in the alpha and beta globin gene clusters [35,36] and between the lysozyme and Gas41 genes [18,19]. It is also interesting to note that the origin in the Mcm4-Prkdc intergenic region has been conserved between between chickens and humans.
Another interesting aspect of our work is the identification of the chicken Mcm4 gene as a Myb-regulated gene. MCM4, together with other MCM proteins, forms the hex-Replication origin activity of the promoter region of the chicken adenosine receptor 2B gene Figure 6 Replication origin activity of the promoter region of the chicken adenosine receptor 2B gene. A. Schematic illustration of the chicken A2B receptor gene. The black boxes show the two exons of the gene. The transcriptional start site is marked by an arrow. The position of primer pairs used in the PCR analysis is shown below. The scale at the top is in kilobases. B. Nascent strand abundance assay of the adenosine receptor 2B promoter region using the PCR primer pairs indicated at the bottom. The columns show the average abundance of the corresponding sequences in nascent strand preparations of BM2 (black columns) or HD11 (white columns) cells relative to the sequences amplified with the -3.9 kb primer pair. Error bars mark the standard deviations. C. Nascent strand abundance analysis of the promoter region using wild-type (black columns) or c-myb deficient (white columns) DT40 cells. americ MCM2-7 complex which functions as helicase during the S-phase of the cell cycle. The MCM2-7 complex is loaded onto the DNA prior to the initiation of DNA replication and constitutes an essential part of the pre-replication complex [for reviews see: [37][38][39]]. In addition to acting as a helicase in concert with the other MCM proteins forming the MCM2-7 complex there is evidence that MCM4 also performs a specific regulatory role. MCM4 is extensively phosphorylated when cells are exposed to inhibitors of DNA synthesis or to UV irradiation, leading to a block of DNA fork progression [40,41], suggesting that MCM4 plays a role in the DNA replication block checkpoint system. In the normal course of the cell cycle MCM4 is phosphorylated by Cdk2, leading to the inhibition of the helicase function of the MCM complex [42,43]. Blockage of this phosphorylation leads to reinitiation of replication and genomic instability. These observations suggest that MCM4 is a key component of the MCM complex whose manipulation can influence the control of DNA replication and genomic stability. Interestingly, elevated Mcm4 mRNA levels have been found in certain tumors, suggesting that increased Mcm4 expression can contribute to the malignant properties of the tumor cells [44,45]. Recently, a hypomorph allele of mouse Mcm4 was identified and shown to cause chromosomal instability [46]. Thus, Mcm4 is an interesting novel Myb target gene that provides a possible link between Myb, DNA-replication and checkpoint control.

Conclusion
We have identified the chicken Mcm4 gene as a novel Myb-regulated gene and shown that the Mcm4 promoter region is associated with an origin of DNA replication. Similarly, our work shows that the promoter of the Mybregulated adenosine receptor 2B gene co-localizes with a replication origin. Our work raises the possibility that a fraction of Myb target gene promoters is associated with DNA replication origins.

Cell culture
The BM2 and HD11 are lines of AMV-transformed chicken myeloblasts and MC29-transformed macrophages that were originally obtained from C. Moscovici and T. Graf, respectively. BM2 cells were grown in RPMI 1640 medium supplemented with 5 % fetal calf serum, 5 % chicken serum and 10 % tryptone phosphate broth (Gibco). HD11 cells were grown in basal Iscoves' medium supplemented with 8% fetal calf serum and 2% chicken serum. 10.4 is a derivative of the HD11 cell line expressing a v-Myb/ER fusion protein [25]. 10.4 cells were grown in the same medium as HD11 cells. To activate the v-Myb/ER fusion protein the growth medium was supplemented with 2 µM β-estradiol for 24 hours.

Northern blotting
Preparation of polyadenylated RNA and Northern blotting were performed as described [25]. Mcm4 and Prkdc mRNAs were detected using cDNA clones of chicken Mcm4 (riken1_3305r1) and Prkdc (riken1_9d19r2), kindly provided by H. Arakawa and J.M. Buerstedde. As an internal control a specific probe for the ribosomal S17 gene was used.

Electrophoretic mobility shift assay
The following pairs of single-stranded oligonucleotides were annealed and used for electrophoretic mobility shift assays: MCM4-MBS-1_sense: After annealing, the oligonucleotides were radiolabeled by filling-in the ends using Klenow polymerase and α 32 P-dCTP. Preparation of bacterial full-length v-Myb protein and binding experiments were performed as described [47].

Chromatin immunoprecipitation
Approximately 10 8 BM2 cells were incubated in 1/10 cross-linking solution containing 0.1 M NaCl; 1 mM EDTA; 0.5 mM EGTA; 50 mM Tris-HCl, pH 8.0 and 11% formaldehyde for 10 min and quenched for 5 min in 125 mM glycine. After washing in ice-cold phosphate-buffered saline, cells were treated for 20 min each with washing solution A (0.25% Triton X100; 10 mM Tris-HCl, pH8.0, 10 mM EDTA; 0.5 mM EGTA) and B (200 mM NaCl; 10 mM Tris-HCl, pH8.0; 1 mM EDTA; 0.5 mM EGTA). Nuclei were sonicated on ice (4 times for 20 sec in 2 min intervals) in egg lysis buffer (ELB) (120 mM NaCl; 50 mM Tris-HCl, pH7.5; 20 mM NaF; 1 mM EDTA; 6 mM EGTA; 15 mM sodiumpyrophosphate; 1 mM phenylmethylsulfonyl fluoride and 0.1% Nonidet P-40). DNA-protein complexes were incubated with two different Myb-specific antibodies raised against the DNA-binding domain of v-Myb [48], normal rabbit serum or anti-Flag antibody (Sigma). After precipitation with protein-A-sepharose the samples were washed several times in ELB buffer. Following elution with 0.5% SDS the DNA was recovered by reverse-crosslinking for 6 h at 37°C and 65°C, respectively, in a buffer containing 0.5% SDS; 10 mM DTT and 100 µg proteinase K. The immunoprecipitated DNA was then purified by phenol-chloroform extraction and ethanol precipitation. PCR-amplification was performed by quantitative real-time PCR using primers specific for the chicken MCM4 promoter region (-0.55 kb) or primers derived from the -13 kb region of the gene.

Nascent strand abundance assay
Analysis of replication origin activity was performed according to Giacca et al. [26]. Total genomic DNA was isolated from approximately 10 8 exponentially growing HD11 or BM2 cells using the Nucleobond CB kit (Macherey & Nagel) following the manufacturer's instructions. DNA was resuspended in TNE buffer (10 mM Tris, pH 8.0; 100 mM NaCl; 1 mM EDTA) and heat-denatured for 7 min at 95°C followed by rapid cooling on ice. Denatured DNA was layered on a linear neutral sucrose gradient (5-30% in TNE) and size fractionated by centrifugation in a Beckman SW28 rotor at 26.000 rpm for 20 h at 20°C. Nascent strand DNA fractions with an average DNA size of 1-1.5 kb were pooled, precipitated with ethanol and and treated with T4-polynucleotide kinase and λ-exonuclease as described previously [49,50]. The purified nascent DNA strands were analyzed by quantitative real-time PCR using the following primer pairs: Publish with Bio Med Central and every scientist can read your work free of charge