Transcriptional inhibiton of Hoxd4 expression by miRNA-10a in human breast cancer cells

Background Small noncoding RNAs (ncRNAs), including short interfering RNAs (siRNAs) and microRNAs (miRNAs), can silence genes at the transcriptional, post-transcriptional or translational level [1,2]. Results Here, we show that microRNA-10a (miR-10a) targets a homologous DNA region in the promoter region of the hoxd4 gene and represses its expression at the transcriptional level. Mutational analysis of the miR-10a sequence revealed that the 3' end of the miRNA sequence is the most critical element for the silencing effect. MicroRNA-10a-induced transcriptional gene inhibition requires the presence of Dicer and Argonautes 1 and 3, and it is related to promoter associated noncoding RNAs. Bisulfite sequencing analysis showed that the reduced hoxd4 expression was accompanied by de novo DNA methylation at the hoxd4 promoter. We further demonstrated that trimethylation of histone 3 lysine 27 (H3K27me3) is involved in the miR-10a-induced hoxd4 transcriptional gene silence. Conclusion In conclusion, our results demonstrate that miR-10a can regulate human gene expression in a transcriptional manner, and indicate that endogenous small noncoding RNA-induced control of transcription may be a potential system for expressional regulation in human breast cancer cells.


Conclusion:
In conclusion, our results demonstrate that miR-10a can regulate human gene expression in a transcriptional manner, and indicate that endogenous small noncoding RNAinduced control of transcription may be a potential system for expressional regulation in human breast cancer cells.

Background
MicroRNAs (miRNAs) are an important small noncoding family of 19 to 26 nucleotide long endogenous RNAs that play critical roles in cognate mRNA cleavage and translational repression [1,2]. They participate in a variety of cell physiological functions such as metabolism, differentiation, morphogenesis, development and apoptosis [3]. Large numbers of miRNA have been identified in almost all genetically dissected species including animals, plants, and viruses (miRBase Release 12.0). Experimental evidence implies that miRNAs can regulate tumor susceptibility genes [4,5], and expression profiling assays have uncovered characteristic miRNA signatures in human tumors [6,7]. MicroRNA-induced transcriptional gene silencing through de novo DNA methylation or chromatin modification has been demonstrated in yeast and plants [8]. Although it has been reported that exogenous siRNAs can mediate Page 1 of 9 (page number not for citation purposes)

BioMed Central
Open Access transcriptional inhibition through promoter methylation in human cells and miRNA could act as a cis-regulator to modulate gene expression [9][10][11], transcriptional inhibition directed by endogenous small noncoding RNAs remains to be reported.
Homeobox genes are a group of evolutionarily conserved members that regulate animal morphological diversity at the organismal and evolutionary level [12]. Computational analyses have identified most vertebrate and invertebrate Hox genes as putative miRNA targets. It is believed that knowledge about the relationship between Hox genes and miRNAs is important for the understanding of the Hox gene regulatory mechanism in animal development as well as in tumor invasion and metastasis [13,14]. The human hsa-miR-10a locus maps upstream of hoxb4 and the hsa-miR-10b locus is similarly situated in the promoter region of hoxd4 (Fig. 1a). Hsa-miR-10a and hsa-miR-10b deviate in only one nucleotide located at the center of their sequence, and the miR-10 family exhibits strong evolutionary conservation across a number of animal species such as human, mouse, zebrafish, Drosophila/fly and chicken (Fig. 1b). The zebrafish miR-10a and miR-10b loci are not always coordinately expressed with their downstream hox gene suggesting that they have independent transcriptional initiation systems [14]. When expressed, the primary hsa-miR-10b transcript would be equivalent to a promoter-associated RNA [15] which could be targeted by miR-10a and thereby mediate induction of transcriptional gene silence of the hoxd4 locus. Here, we show that an endogenous miRNA transcriptionally modulates hoxd4 expression in human cancer cells through de novo DNA methylation.

MicroRNA-10a inhibits hoxd4 gene expression
To explore whether miR-10a can suppress hoxd4 expression, the expression levels of the hoxd4 and hoxb4 mRNAs were compared to those of miR-10a and -10b in several human cell lines including human breast cancer cells MDA-MB-231 and MCF7, human mammary epithelial cells (MCF10A), hepatocellular liver carcinoma cells (HepG2), cervical carcinoma cells (HeLa) and lung adenocarcinoma cells (A549) (Fig. 1c, d). The hoxd4 expression is high in MDA-MB-231 cells, but low in MCF-7 cells and MCF10A cells (Fig. 1d). This expression pattern is similar to that of the adjacent miR-10b locus, and is consistent with the possibility that these two loci are coordinately regulated (Fig. 1c). The expression of miR-10a is higher than of miR-10b in MCF7 cells, but lower than the miR-10b expression in MDA-MB-231 cells (Fig. 1c), and the expression of miR-10a varies inversely with the expression of hoxd4 in all six cell types.
This negative correlation indicates that miR-10a might play a role in modulating hoxd4 gene expression in these cells. The observation that miRNAs 10a and 10b do not always present similar expression profiles as their adjacent genes resemble similar findings in zebrafish  [14] and could indicate that under some conditions the miRNAs may be regulated independently of their adjacent hox genes (Fig. 1c, d).
To establish that miR-10a inhibits hoxd4 gene expression, we first performed in vitro loss-of-function analyses by silencing the miRNA with antisense oligonucleotides and assessing hoxd4 gene expression. Quantitative PCR showed that unlike negative control 2'-O-methyl oligos (N.C.) or anti-miR-196a, the transfection of a modified 2'-O-methyl miR-10a antisense oligonucleotide (anti-miR-10a) resulted in a 2-fold increase in hoxd4 mRNA levels MDA-MB-231 cells and a 4-fold increase in hoxd4 mRNA levels in MCF7 cells (Fig. 1e). No change was observed in the expression levels of the adjacent hoxd3 and hoxd8 loci (Fig. 1f), indicating that the miR-10ainduced hoxd4 gene suppression did not affect other regions on the same chromosome. Nor was there any change in hoxd4 protein expression levels when MDA-MB-231 cells were transfected with negative control 2'-Omethyl oligos (N.C.) or anti-miR-196a (Fig. 1g), suggesting the observed increase in hoxd4 expression is a specific effect of the endogenous miR-10a.

MicroRNA-10a decreases hoxd4 gene expression through transcriptional inhibition
To determine whether miR-10a over-expression would influence the hoxd4 mRNA expression level, 100 nM miR-10a duplex was transfected into MCF7 and MDA-MB-231 cells (Fig. 2a). In both cell lines, the result was a five to eight fold decrease in the hoxd4 mRNA levels 48 hrs after transfection. Western blot analysis of MDA-MB-231 cells showed that the Hoxd4 protein levels were consistent with the results obtained with qPCR ( Fig. 2b). It has been reported that miR-10a could target the 3'UTR sequence of the hoxd10 gene [13], but based on current algorithms (miRanda) there are no likely miR-10a target sites in the 3'UTR of the hoxd4 mRNA. We assessed the levels of miRNA silencing by cloning the 3'UTRs of these two genes into a luciferase reporter. We found that transfection of 100 nM miR-10a duplexes into MCF7 cells caused a 50% decrease in luciferase activity in the reporter containing the hoxd10 3'UTR while no changes was detected in cells expressing the reporter with the hoxd4 3'UTR ( Fig. 2c). However, upstream of the hoxd4 transcription initiation site there is a site with near perfect complementarity to the miR-10a sequence. An intriguing question is therefore whether miR-10a could regulate hoxd4 gene expression in a transcriptional manner. To establish that the loss of hoxd4 expression was due to transcriptional silencing by miR-10a, we carried out nuclear run-on experiments (Fig. 2d). Hoxd4 transcription was almost abolished in MCF7 nucleii when treated with miR-10a duplexes, whereas no changes were observed when MCF7 was transfected with the siRNA sid4 designed to target the hoxd4 mRNA for cleavage. When MCF7 cytoplasmic extracts were treated similarly, both miR-10a and sid4 significantly reduced hoxd4 mRNA levels, suggesting that the miR-10a To test whether miR-10a-induced suppression of hoxd4 mRNA is common in other human cell lines, MCF10A, HepG2, HeLa and A549 were also investigated. All six cell types showed a similar tendency towards reduced hoxd4 expression when transfected with 100 nM miR-10a (Fig. 2e). To determine which part of the miRNA sequence is most important for the observed effects, we mutated the miR-10a sequence in either the first (or 5'most) five basepairs (G5'), in the middle five basepairs (M), or in the last (or 3'most) five basepairs (G3'), and analyzed the effects of the mutant miR-10a duplexes in MCF7 cells (Fig. 2f). Transfection with miR-10a-G5' and miR-10a-M inhibited hoxd4 mRNA expression to the same extent as did wildtype miR-10a, however, transfection with miR-10a-G3' produced nearly no inhibition of the hoxd4 mRNA expression (Fig. 2g). These data indicate that miR-10a specifically decrease hoxd4 expression through transcriptional inhibition, and suggest that the 3'most portion of miR-10a is most important for this activity.
MicroRNA-10a-induced transcriptional inhibition of hoxd4 is related to promoter-associated ncRNAs The human miR-10a and miR-10b sequences deviate in only one nucleotide, and the mature miR-10a could in principle regulate hoxd4 by targeting the primary miR-10b transcript. Expression of intergenic ncRNA loci has been shown to influence the expression of other, nearby loci in the human genome, and several noncoding RNAs have been detected upstream of hoxd4 locus [15,16]. To assess whether miRNA-induced transcriptional inhibition of the hoxd4 locus depends on the target site being located within a promoter-associated transcript [15], several RNA duplexes complementary to the hoxd4 promotor were designed. The duplexes were designed to target nc-hoxd4-34, nc-hoxd4-35, or nc-hoxd4-36 loci [17] (si-nc34, si-nc35, si-nc36, respectively) or the nontranscribed sequence spacing these loci (siD1, siD2) (Fig. 1a). In accordance with the idea that RNA expression is required for promoter targeting, we found that two of the siRNAs targeting ncRNA loci (si-nc34 and si-nc36) in MCF7 cells inhibited hoxd4 expression, whereas the siRNAs targeting the intervening nontranscribed sequence siD1 and siD2 had almost no effect in hoxd4 expression (Fig. 3a). Further analysis of the effect of the siRNAs showed that both si-nc34 and si-nc36 clearly reduced the levels of their respective noncoding transcript targets (Fig. 3b), whereas si-nc35 did not affect the expression level of nc-hoxd4-35, possibly also explaining the failure of this siRNA to affect hoxd4 expression. To explore whether the secondary structure of the promoter-associated RNA target would influence the observed transcriptional inhibition, two RNA duplexes was designed to target the pri-miR-10b transcript either at the stem region (siP1) or the loop region (siP2; Fig. 3c  decreased the expression of hoxd4 even more strongly than did the miR-10a duplex, whereas transfection with the siP2 duplex had no affect on the hoxd4 expression (Fig. 3d). The data thus suggest that miRNA-induced transcriptional inhibition is mediated through interference with promoter-associated transcripts, and that the secondary structure of the promoter-associated ncRNAs can influence the inhibitory effect.

MiR-10a/b do not silence transcription of immediate downstream genes
If the transcriptional inhibition of hoxd4 expression is achieved by miR-10a targeting the miR-10b primary transcript, then the possibility remains that it could also target its own primary transcript. To assess this possibility and also whether miR-10b might target the primary miR-10a transcript and thereby induce transcriptional inhibition its adjacent hoxb4 locus, the hoxd4 and hoxb4 mRNA levels in MCF7 and MDA-MB-231 cells were evaluated by quantitative RT-PCR. The results show that hoxd4 expression was reduced when transfected with the miR-10a duplexes, and similarly that hoxb4 was downregulated after transfection with miR-10b. Neither miR-10a nor miR-10b reduced the expression of their respective immediate downstream hox locus, possibly indicating that none of these miRNAs are able to target their own primary transcripts (Fig. 3e). MiR-10a/b and miR-320 are both encoded in the promoter region of genes, and the observation that miR-320 has as a cisregulatory role may be very important in miRNA research [11]. Although, there are no negative correlations between the expression of the miRNAs and their respective host genes, and miRNAs target their homologous pre-miRNA sites to modulate gene expression would be also another interesting research topic in molecular biology.

Dicer and AGO1/3 proteins are involved in miR-10a induced transcriptional gene inhibition
Previous studies have shown that siRNAs targeting selected promoter regions of human genes require the recruitment of the Argonaute 1 (Ago1) and Argonaute 2 (Ago2) [18,19]. Also knockdownofDicer relieve the transcriptional inhibition and attenuate abnormal promoter methylation in human cells [20]. We decided to test whether transcriptional inhibition induced by endogenous small noncoding RNAs is Ago-dependent, and also whether Dicer is involved in this process. Specific siRNAs targeting Agos 1-4 (siAgo1, siAgo2, siAgo3 and siAgo4) and Dicer (siDicer) were shown to knock-down the corresponding Argonaute and Dicer mRNA efficiently [19,21]. Each of these siRNAs was transfected into MCF7 cells, either alone or in combination with the miR-10a duplex. Co-transfected with the miR-10a duplex, siAgo1 or siDicer almost completely prevented the reduction in hoxd4 expression induced by miR-10a (Fig. 3f). SiAgo3 produced a similar, but less pronounced effect. These data show that AGO1 and AGO3 are required for the induction of transcriptional inhibition by endogenous small noncoding RNAs. The effect of Dicer knockdown further suggests involvement of this protein in DNA methylation of the hoxd4 promotor.
MicroRNA-10a-induced inhibition of transcription is associated with DNA methylation of the hoxd4 promotor Transcriptional silencing is commonly associated with epigenetic chromatin modifications. To test whether miR-10a suppresses hoxd4 expression by de novo DNA methylation, MCF7 and MDA-MB-231 cells were treated for 5 days with 0.75 μM 5-Aza-2'-deoxycytidine (5-aza-dC), a DNA methylotransferase (DNMT) inhibitor. In MCF7 cells, the 5-aza-dC treatment clearly upregulated the expression of the p15 gene, whose promotor is normally methylated, but did neither affect the expression of the unmethylated gapdh gene nor affect the expression of miR-10a (Fig. 4a). Treatment with 5-aza-dC increased the hoxd4 mRNA level about 5-fold in MCF7 cells, compared to only 2-fold in MDA-MB-231 cell lines for 5 days (Fig. 4b).
To show that miR-10a can silence hoxd4 expression by promoter methylation, MCF7 cells and MDA-MB-231 cells were transfected with either the miR-10a duplexes or anti-miR-10a, and then treated with 1 μM 5-aza-dC for 48 h. We found that after inhibiton of the DNA methylotransferase activity, miR-10a could no longer silence hoxd4 mRNA expression (Fig. 4c).
Targeting promoters with siRNAs have been shown to relative with the facultative heterochromatin marks H3K9me2 and H3K27me3 in human cells [9,18]. To determine whether miR-10a could induce these repressive histone modifications, we used chromatin immunoprecipitation (ChIP) to screen the hoxd4 promoter for H3K9me2 and H3K27me3 at regions overlapping the miR-10a target site (Chip Box1: -1081~-972) and the bisulphite sequencing PCR (BSP) detection site (Chip Box2: -330~-122). Western blot analysis demonstrated the presence of H3K9me2 and H3K27me3 in the promoter region of the hoxd4 gene (Fig. 4d). After transfection with miR-10a, an increase in H3K27me3 in the two analysed regions (Box1 and Box2) was observed in both MCF7 cells and MDA-MB-231 cells (Fig. 4e). We next analysed the DNA methylation status by performing bisulphite sequencing of the hoxd4 promoter. Aberrant methylation is often observed in cancer cells. CpG and CpNpG methylation are both strongly correlated with gene silencing in plants. CpNpG methylation is rare in the human genome, and no functional implications for BMC Molecular Biology 2009, 10:12 http://www.biomedcentral.com/1471-2199/10/12 gene silencing have ever been documented [22]. Consistents with previous studies [22,23], we found no CpNpG methylation in the our bisulfite-sequenced region (BSP Sequencing Region: -330~-122). Cultures treated with mock duplexes presented CpG methylation in this region, but consistent with the observation that the hoxd4 mRNA level is lower in MCF7 than in MDA-MB-231 cell lines, the hoxd4 promoter region was clearly more densely methylated in MCF7 cells than in MDA-MB-231 cells. When the cultures were transfected with miR-10a duplexes, the extent of methylation increased compared to cultures treated with control RNA duplexes. Contrarily, when cells were treated with 2'-O-methyl-miR-10a (anti-miR-10a), we found that the extent of methylation decreased and was accompanied by upregulation hoxd4 expression in both cells types (Fig. 4f).
These results thus indicate that the miR-10a-induced inhibition of hoxd4 transcription is accompanied by de novo DNA methylation and H3K27me3 formation in the targeted promoter region.

Discussion
We demonstrate that an endogenous small noncding RNA involved in transcriptional gene regulation in human cells. This observation gives new insights into the mechanisms by which an increase in the miR-10a expression leads to a concomitant reduction in the hoxd4 expression. Bidirectional transcription leading to two functionally different miRNAs originating from the same genomic locus has been reported in flies [24][25][26]. Here, we show that two miRNAs, miR-10a and miR-10b, mapping to two different chromosomes, can target each other's primary transcipts to repress the expression of neighboring hox genes. Antisense RNA-mediated gene silencing and de novo methylation of CpG islands in the promoter have been demonstrated in humans [27]. However, the molecular mechanisms by which small RNAs mediate DNA methylation of targeted promoter regions in the human genome remains to be elucidated. Some groups have observed siRNA-directed DNA methylation at targeted promoters [9,10,28,29] whereas others have not found such an effect [19,22]. A recent report has showed that miR-10a could bind the 5'UTR of ribosomal protein mRNAs and enhance their translation [30]. One possible explanation for this observation might that non-coding RNAs are expressed in the target region. It has been demonstrated that promoter-associated noncoding RNAs can be recognized by miRNAs and direct epigenetic silencing complexes to the corresponding targeted promoters, thereby mediating transcriptional inhibition [15]. In the case of the hoxd4 locus, the adjacent pri-miR-10b could serve as a promoter-associated non-coding RNA, mediating induction of transcriptional silencing when targeted by miR-10a.
Recently, Dicer has been reported to attenate abnormal promoter DNA methylation in cancer cells [20]. Our results define the first case in which an endogenous miRNA targets and methylates a promoter region through Dicer action. In plants, methylation is a crucial step in microRNA biogenesis, and depends on HEN1, a methyltransferase that adds a methyl group to the 3'most nucleotide of small non-coding RNAs in both plants and mammals [31,32]. miRNAs that regulate Rbl2-dependent DNMT expression in mouse embryonic stem cells have also been detected [29,33]. Ago1 and Ago4 has been shown to be involved in siRNA-directed chromatin modification, including histone methylation and non-CpG DNA methylation in plants and yeast [34,35]. Ago1 is required for both siRNA-mediated transcriptional gene silencing and the recruitment of histone methyltransferase activity to H3K9me2 and H3K27me3 at a siRNA-targeted promoters in human cells [18], and Ago3 has the ability to interact with methyltransferases [36]. Here we show that Ago 1 and Ago3 participate in miRNA-mediated de novo DNA methylation in human cancer cells, further implicating both the miRNA machinery and the chromatin remodelling complexes in RNA directed transcriptional gene silencing.

Conclusion
Taken together, our data support the notion that an RNA-operated system is involved in transcriptional regulation of genes in human cells. Endogenous small non-coding RNAs might control or fine-tune gene expression at both the transcriptional and post-transcriptional levels. An understanding of such a regulatory system could prove valuable in targeted approaches to specific control of gene expression and treatment of human cancers.

RNA isolation and miRNA detection
Total RNA from cultured cells was isolated using the mirVana miRNA Isolation Kit (Ambion). Detection of the mature form of miRNAs was performed using the Hairpin-it miRNAs qPCR Quantitation Assay, according to the manufacturer's instructions (GenePharma). The U6 small nuclear RNA was used as an internal control.

Western blotting
Total protein (40 μg) was resolved with 10% SDSpolyacrylamide gel eletrophoresis and bands of protein transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham). The membrane was blocked with 5% nonfat milk TBS buffer overnight at RT, and incubated for 2 hours with primary antibodies. β-actin was used as loading control. The antibodies used included hoxd4 (Abcam), β-actin (SantaCruz Biotechnology). The membranes then were incubated for 1 h with HRP-conjugated goat anti-mouse (Zymed Laboratories) or rabbit anti-goat (SantaCruz Biotechnology) secondary antibody. Immunocomplexes were visualized with an ECL kit (Pierce).

Methylation analysis and sodium bisulphite DNA sequencing
Genomic DNA (1 μg) was treated with sodium bisulphate and. used to PCR-amplify the hoxd4 promoter regions (-300 bp -122 bp). The amplifed product was purified using a Qiagen PCR purification kit (Qiagen) and sequenced using the sense primer with an ABI automated fluorescent sequencer according to the manufacturer's instructions.

qRT-PCR
We extracted total RNA from the treated cells with Trizol (Invitrogen) and treated it with DNase (Qiagen). We carried out qRT-PCR analysis using QuantiTeck SYBR Green PCR kit (Qiagen). PCR primers used in this paper are listed in Table 1, Additional file 1. Real-time PCR was performed with the LightCycler 2.0 (Roche).
Nuclear run-on assay Nuclear run-on assays were performed in accordance with the Current Protocols of Molecular Biology. Digoxinum linked dUTP was added to an in vitro transcription reaction and precipitated with digoxinum antibody. Amplified β-actin served as a loading control. Bound RNA was eluted from the beads by adding Trizol (Invitrogen) to the beads, followed by RNA extraction and RT-real time PCR as described previously.

Chromatin immunoprecipitation
We cross-linked DNA and processed it in accordance with the UpState Chromatin Immunoprecipitation (ChIP) Assay Kit protocol (UpState 17-295). We used 2 × 10 6 cells for each immunoprecipitation reaction, and used rabbit antibody to H3K27me3 (Upstate 07-449) for specific immunoprecipitation of the histone residues. The precipitates were analyzed by real-time PCR with two sets of hoxd4 promoter specific primers spanning the CpG island of interest (Table 1, Additional file 1).
Luciferase reporter assay Cells of 50% confluence in 24-well plates were transfected using Lipofectamine 2000 (Invitrogene). Firefly luciferase reporter gene constructs (200 ng) and pRL-SV40 Renilla luciferase construct (1 ng; for normalization) were cotransfected per well. Cell extracts were prepared 48 h after transfection, and the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega).

Statistical Analysis
All experiments were repeated 3 times. Data are presented as mean ± s.e.m. Student's t test (two-tailed) was used to compare two groups (P < 0.05 was considered significant). Two asterisks indicated that the p-value was less than 0.01, one asterisks indicated that the p-value was less than 0.05.