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  1. Content type: Research article

    In continuing our research into the new family of bifunctional restriction endonucleases (REases), we describe the cloning of the tsoIRM gene. Currently, the family includes six thermostable enzymes: TaqII, Tth11...

    Authors: Piotr M Skowron, Jolanta Vitkute, Danute Ramanauskaite, Goda Mitkaite, Joanna Jezewska-Frackowiak, Joanna Zebrowska, Agnieszka Zylicz-Stachula and Arvydas Lubys

    Citation: BMC Molecular Biology 2013 14:17

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  2. Content type: Research article

    The RNA binding protein DEAD-END (DND1) is one of the few proteins known to regulate microRNA (miRNA) activity at the level of miRNA-mRNA interaction. DND1 blocks miRNA interaction with the 3′-untranslated reg...

    Authors: Sara Ali, Namrata Karki, Chitralekha Bhattacharya, Rui Zhu, Donna A MacDuff, Mark D Stenglein, April J Schumacher, Zachary L Demorest, Reuben S Harris, Angabin Matin and Sita Aggarwal

    Citation: BMC Molecular Biology 2013 14:16

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  3. Content type: Research article

    The co-activator-associated arginine methyltransferase 1 (CARM1) catalyzes the methylation of HuR. However, the functional impact of this modification is not fully understood. Here, we investigated the influen...

    Authors: Lijun Pang, Haiyan Tian, Na Chang, Jie Yi, Lixiang Xue, Bin Jiang, Myriam Gorospe, Xiaowei Zhang and Wengong Wang

    Citation: BMC Molecular Biology 2013 14:15

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  4. Content type: Research article

    Tip110 plays important roles in tumor immunobiology, pre-mRNA splicing, expression regulation of viral and host genes, and possibly protein turnover. It is clear that our understanding of Tip110 biological fun...

    Authors: Khalid Amine Timani, Ying Liu and Johnny J He

    Citation: BMC Molecular Biology 2013 14:14

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  5. Content type: Research article

    Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids). Flavonoid biosynthesis was studied int...

    Authors: Ellen De Keyser, Laurence Desmet, Erik Van Bockstaele and Jan De Riek

    Citation: BMC Molecular Biology 2013 14:13

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  6. Content type: Research article

    Ribosomal protein L30 belongs to the L7Ae family of RNA-binding proteins, which recognize diverse targets. L30 binds to kink-turn motifs in the 28S ribosomal RNA, L30 pre-mRNA, and mature L30 mRNA. L30 has a n...

    Authors: Abby L Bifano, Tarik Atassi, Tracey Ferrara and Donna M Driscoll

    Citation: BMC Molecular Biology 2013 14:12

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  7. Content type: Research article

    RA (retinoic acid) signal pathway has been proved to be required for germ cell meiotic initiation in mammals, aves and amphibians. Stra8 (Stimulated by retinoic acid gene 8) is an important factor in RA signal...

    Authors: Ranran Dong, Shijie Yang, Jing Jiao, Tingru Wang, Hongjuan Shi, Linyan Zhou, Yaoguang Zhang and Deshou Wang

    Citation: BMC Molecular Biology 2013 14:11

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  8. Content type: Research article

    A member of the NF-κB signaling pathway, PoAkirin1, was cloned from a full-length cDNA library of Japanese flounder (Paralichthys olivaceus). The full-length cDNA comprises a 5′UTR of 202 bp, an open reading fram...

    Authors: Chang-Geng Yang, Xian-Li Wang, Bo Zhang, Bing Sun, Shan-Shan Liu and Song-Lin Chen

    Citation: BMC Molecular Biology 2013 14:10

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  9. Content type: Review

    The double-stranded conformation of cellular DNA is a central aspect of DNA stabilisation and protection. The helix preserves the genetic code against chemical and enzymatic degradation, metabolic activation, ...

    Authors: Nicholas W Ashton, Emma Bolderson, Liza Cubeddu, Kenneth J O’Byrne and Derek J Richard

    Citation: BMC Molecular Biology 2013 14:9

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  10. Content type: Research article

    MicroRNAs (miRNAs) are a type of non-coding small RNA ~22 nucleotides in length that regulate the expression of protein coding genes at the post-transcriptional level. Glycolytic and oxidative myofibers, the t...

    Authors: Yingkai Liu, Mingzhou Li, Jideng Ma, Jie Zhang, Chaowei Zhou, Tao Wang, Xiaolian Gao and Xuewei Li

    Citation: BMC Molecular Biology 2013 14:7

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  11. Content type: Methodology article

    The ability to interrogate circulating tumor cells (CTC) and disseminated tumor cells (DTC) is restricted by the small number detected and isolated (typically <10). To determine if a commercially available tec...

    Authors: Christopher J Welty, Ilsa Coleman, Roger Coleman, Bryce Lakely, Jing Xia, Shu Chen, Roman Gulati, Sandy R Larson, Paul H Lange, Bruce Montgomery, Peter S Nelson, Robert L Vessella and Colm Morrissey

    Citation: BMC Molecular Biology 2013 14:6

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  12. Content type: Research article

    Apolipoprotein B (APOB) is an integral component of the chylomicron and the atherogenic lipoproteins LDL and Lp(a). Exon 26 of the APOB pre-mRNA is unusually long at 7,572 nt and is constitutively spliced. It is ...

    Authors: Umasuthan Srirangalingam, Scott A Akker, Dennis Norman, Naveenan Navaratnam, Shern L Chew and Bernard Khoo

    Citation: BMC Molecular Biology 2013 14:5

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  13. Content type: Research article

    T-cell intracellular antigen (TIA) proteins function as regulators of cell homeostasis. These proteins control gene expression globally at multiple levels in response to dynamic regulatory changes and environm...

    Authors: Carmen Sánchez-Jiménez, Isabel Carrascoso, Juan Barrero and José M Izquierdo

    Citation: BMC Molecular Biology 2013 14:4

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  14. Content type: Research article

    Duchenne muscular dystrophy (DMD) is the most common X-linked muscle degenerative disease and it is due to the absence of the cytoskeletal protein dystrophin. Currently there is no effective treatment for DMD....

    Authors: Annalisa Onori, Cinzia Pisani, Georgios Strimpakos, Lucia Monaco, Elisabetta Mattei, Claudio Passananti and Nicoletta Corbi

    Citation: BMC Molecular Biology 2013 14:3

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  15. Content type: Research article

    Pseudorabies virus (PRV), an alpha-herpesvirus of swine, is a widely used model organism in investigations of the molecular pathomechanisms of the herpesviruses. This work is the continuation of our earlier st...

    Authors: Irma F Takács, Dóra Tombácz, Beáta Berta, István Prazsák, Nándor Póka and Zsolt Boldogkői

    Citation: BMC Molecular Biology 2013 14:2

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  16. Content type: Research article

    In Drosophila embryos, checkpoints maintain genome stability by delaying cell cycle progression that allows time for damage repair or to complete DNA synthesis. Drosophila MOF, a member of MYST histone acetyl tra...

    Authors: Sreerangam NCVL Pushpavalli, Arpita Sarkar, M Janaki Ramaiah, Debabani Roy Chowdhury, Utpal Bhadra and Manika Pal-Bhadra

    Citation: BMC Molecular Biology 2013 14:1

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  17. Content type: Research article

    Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and def...

    Authors: Victoria A Fleming, Cuiyu Geng, Andrea N Ladd and Hua Lou

    Citation: BMC Molecular Biology 2012 13:35

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  18. Content type: Research article

    Autonomously Replicating Sequences (ARS) in S. cerevisiae serve as origins of DNA replication or as components of cis-acting silencers, which impose positional repression at the mating type loci and at the telome...

    Authors: Patricia Chisamore-Robert, Samantha Peeters, Kristina Shostak and Krassimir Yankulov

    Citation: BMC Molecular Biology 2012 13:34

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  19. Content type: Research article

    Defects in the human BLM gene cause Bloom syndrome, notable for early development of tumors in a broad variety of tissues. On the basis of sequence similarity, BLM has been identified as one of the five human hom...

    Authors: Michael W Killen, Dawn M Stults, William A Wilson and Andrew J Pierce

    Citation: BMC Molecular Biology 2012 13:33

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  20. Content type: Research article

    The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a devastating pest of cruciferous crops worldwide, and has developed resistance to a wide range of insecticides, including diacylhydra...

    Authors: Baozhen Tang, Wei Dong, Pei Liang, Xuguo Zhou and Xiwu Gao

    Citation: BMC Molecular Biology 2012 13:32

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  21. Content type: Research article

    Adenylate kinase 2 (AK2) is a phosphotransferase that catalyzes the reversible reaction 2ADP(GDP) ↔ ATP(GTP) + AMP and influences cellular energy homeostasis. However, the role of AK2 in regulating cell prolif...

    Authors: Ru-Ping Chen, Chun-Yan Liu, Hong-Lian Shao, Wei-Wei Zheng, Jin-Xing Wang and Xiao-Fan Zhao

    Citation: BMC Molecular Biology 2012 13:31

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  22. Content type: Methodology article

    Recombinatorial cloning using the GatewayTM technology has been the method of choice for high-throughput omics projects, resulting in the availability of entire ORFeomes in GatewayTM compatible vectors. The Multi...

    Authors: Astrid Nagels Durand, Tessa Moses, Rebecca De Clercq, Alain Goossens and Laurens Pauwels

    Citation: BMC Molecular Biology 2012 13:30

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  23. Content type: Research article

    The major rate-limiting enzyme for de novo cholesterol synthesis is 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR). HMGCR is sterically inhibited by statins, the most commonly prescribed drugs for the...

    Authors: Camilla Stormo, Marianne K Kringen, Runa M Grimholt, Jens P Berg and Armin P Piehler

    Citation: BMC Molecular Biology 2012 13:29

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  24. Content type: Research article

    GAD65 (Glutamic acid decarboxylase 65 KDa isoform) is one of the most important auto-antigens involved in Type 1 diabetes induction. Although it serves as one of the first injury markers of β-islets, the mecha...

    Authors: Sandeep Singh, Varsheish Raina, Pavithra Lakshminarsimhan Chavali, Taronish Dubash, Sreenath Kadreppa, Pradeep Parab and Samit Chattopadhyay

    Citation: BMC Molecular Biology 2012 13:28

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  25. Content type: Research article

    microRNAs (miRNAs) are shown to be involved in the regulation of circadian clock. However, it remains largely unknown whether miRNAs can regulate the core clock genes (Clock and Bmal1).

    Authors: Xiaochao Tan, Peng Zhang, Lan Zhou, Bin Yin, Hui Pan and Xiaozhong Peng

    Citation: BMC Molecular Biology 2012 13:27

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  26. Content type: Research article

    The human OXR1 gene belongs to a class of genes with conserved functions that protect cells from reactive oxygen species (ROS). The gene was found using a screen of a human cDNA library by its ability to suppr...

    Authors: Kenan C Murphy and Michael R Volkert

    Citation: BMC Molecular Biology 2012 13:26

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  27. Content type: Research article

    It has been reported that the histone deacetylase inhibitor (iHDAc) trichostatin A (TSA) induces an increase in MDR1 gene transcription (ABCB1). This result would compromise the use of iHDACs in combination wi...

    Authors: Trinidad Mata Balaguer, Angeles Gómez-Martínez, Pilar García-Morales, Javier Lacueva, Rafael Calpena, Lourdes Rocamora Reverte, Natividad Lopez Riquelme, Isabel Martinez-Lacaci, José A Ferragut and Miguel Saceda

    Citation: BMC Molecular Biology 2012 13:25

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  28. Content type: Research article

    RNA ligases are essential reagents for many methods in molecular biology including NextGen RNA sequencing. To prevent ligation of RNA to itself, ATP independent mutant ligases, defective in self-adenylation, a...

    Authors: Alexander M Zhelkovsky and Larry A McReynolds

    Citation: BMC Molecular Biology 2012 13:24

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  29. Content type: Research article

    Guanine quadruplex (G4 DNA) is a four-stranded structure that contributes to genome instability and site-specific recombination. G4 DNA folds from sequences containing tandemly repetitive guanines, sequence mo...

    Authors: Edward A Ehrat, Bradley R Johnson, Jonathan D Williams, Glen M Borchert and Erik D Larson

    Citation: BMC Molecular Biology 2012 13:23

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  30. Content type: Research article

    The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The presen...

    Authors: Qingdi Quentin Li, Jeff Skinner and John E Bennett

    Citation: BMC Molecular Biology 2012 13:22

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  31. Content type: Research article

    The enzyme acetyl-CoA carboxylase-alpha (ACC-α) is rate limiting for de novo fatty acid synthesis. Among the four promoters expressing the bovine gene, promoter IA (PIA) is dominantly active in lipogenic tissu...

    Authors: Xuanming Shi, Cornelia C Metges and Hans-Martin Seyfert

    Citation: BMC Molecular Biology 2012 13:21

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  32. Content type: Research article

    Small proline rich protein (SPRR) 2A is one of 14 SPRR genes that encodes for a skin cross-linking protein, which confers structural integrity to the cornified keratinocyte cell envelope. New evidence, however...

    Authors: Yoshiaki Mizuguchi, Susan Specht, John G Lunz III, Kumiko Isse, Natasha Corbitt, Toshihiro Takizawa and Anthony J Demetris

    Citation: BMC Molecular Biology 2012 13:20

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  33. Content type: Research article

    While growing in natural environments yeasts can be affected by osmotic stress provoked by high glucose concentrations. The response to this adverse condition requires the HOG pathway and involves transcriptio...

    Authors: M Gomar-Alba, E Jiménez-Martí and M del Olmo

    Citation: BMC Molecular Biology 2012 13:19

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  34. Content type: Research article

    The vitamin D3 receptor (VDR) is responsible for mediating the pleiotropic and, in part, cell-type-specific effects of 1,25-dihydroxyvitamin D3 (calcitriol) on the cardiovascular and the muscle system, on the ...

    Authors: Christina J Maier, Richard H Maier, Raphaela Rid, Andrea Trost, Harald Hundsberger, Andreas Eger, Helmut Hintner, Johann W Bauer and Kamil Onder

    Citation: BMC Molecular Biology 2012 13:18

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  35. Content type: Research article

    Circulating CD4+ T helper cells are activated through interactions with antigen presenting cells and undergo differentiation into specific T helper cell subsets depending on the type of antigen encountered. In ad...

    Authors: Yan Li, Guobing Chen, Lina Ma, Stephen J Ohms, Chao Sun, M Frances Shannon and Jun Y Fan

    Citation: BMC Molecular Biology 2012 13:16

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  36. Content type: Research article

    We previously defined a family of restriction endonucleases (REases) from Thermus sp., which share common biochemical and biophysical features, such as the fusion of both the nuclease and methyltransferase (MTase...

    Authors: Agnieszka Zylicz-Stachula, Olga Zolnierkiewicz, Arvydas Lubys, Danute Ramanauskaite, Goda Mitkaite, Janusz M Bujnicki and Piotr M Skowron

    Citation: BMC Molecular Biology 2012 13:13

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  37. Content type: Methodology article

    Detection of cell-free methylated DNA in plasma is a promising tool for tumour diagnosis and monitoring. Due to the very low amounts of cell-free DNA in plasma, analytical sensitivity is of utmost importance. ...

    Authors: Inge Søkilde Pedersen, Henrik Bygum Krarup, Ole Thorlacius-Ussing and Poul Henning Madsen

    Citation: BMC Molecular Biology 2012 13:12

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  38. Content type: Research article

    MTG16, MTGR1 and ETO are nuclear transcriptional corepressors of the human ETO protein family. MTG16 is implicated in hematopoietic development and in controlling erythropoiesis/megakaryopoiesis. Furthermore, ...

    Authors: Ram Ajore, Parveen Kumar, Rakesh Singh Dhanda, Urban Gullberg and Inge Olsson

    Citation: BMC Molecular Biology 2012 13:11

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  39. Content type: Research article

    Control of translation allows for rapid adaptation of the cell to stimuli, rather than the slower transcriptional control. We presume that translational control is an essential process in the control of adipog...

    Authors: Carolin Fromm-Dornieden, Silvia von der Heyde, Oleksandr Lytovchenko, Gabriela Salinas-Riester, Bertram Brenig, Tim Beissbarth and Bernhard G Baumgartner

    Citation: BMC Molecular Biology 2012 13:9

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  40. Content type: Research article

    Tristetraprolin (TTP) is a key mediator of processes such as inflammation resolution, the inhibition of autoimmunity and in cancer. It carries out this role by the binding and degradation of mRNA transcripts, ...

    Authors: Magdalena Florkowska, Piotr Tymoszuk, Aleksandra Balwierz, Anna Skucha, Jakub Kochan, Mateusz Wawro, Krystyna Stalinska and Aneta Kasza

    Citation: BMC Molecular Biology 2012 13:8

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  41. Content type: Research article

    The DNA-dependent protein kinase (DNA-PK) is a nuclear complex composed of a large catalytic subunit (DNA-PKcs) and a heterodimeric DNA-targeting subunit Ku. DNA-PK is a major component of the non-homologous e...

    Authors: Birgitte B Olsen, Shih-Ya Wang, Tina H Svenstrup, Benjamin PC Chen and Barbara Guerra

    Citation: BMC Molecular Biology 2012 13:7

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  42. Content type: Research article

    RNA interference (RNAi) and antisense strategies provide experimental therapeutic agents for numerous diseases, including polyglutamine (polyQ) disorders caused by CAG repeat expansion. We compared the potenti...

    Authors: Agnieszka Fiszer, Marta Olejniczak, Pawel M Switonski, Joanna P Wroblewska, Joanna Wisniewska-Kruk, Agnieszka Mykowska and Wlodzimierz J Krzyzosiak

    Citation: BMC Molecular Biology 2012 13:6

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  43. Content type: Research article

    The human papillomavirus (HPV) E2 protein is a multifunctional DNA-binding protein. The transcriptional activity of HPV E2 is mediated by binding to its specific binding sites in the upstream regulatory region...

    Authors: Chen Gao, Ming-Ming Pan, Yan-Jun Lei, Li-Qing Tian, Hui-Ying Jiang, Xiao-Li Li, Qi Shi, Chan Tian, Yu-Kang Yuan, Gui-Xiang Fan and Xiao-Ping Dong

    Citation: BMC Molecular Biology 2012 13:5

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  44. Content type: Research article

    miR-34a functions as an important tumor suppressor during the process of carcinogenesis. However, the mechanism of miR-34a dysregulation in human malignancies has not been well elucidated. Our study aimed to f...

    Authors: Juan Li, Kai Wang, Xuedan Chen, Hui Meng, Min Song, Yan Wang, Xueqing Xu and Yun Bai

    Citation: BMC Molecular Biology 2012 13:4

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