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  1. Content type: Research article

    The stability of reference genes has a tremendous effect on the results of relative quantification of genes expression by quantitative polymerase chain reaction. Equine Inflammatory Airway Disease (IAD) is a c...

    Authors: Laura Beekman, Triin Tohver, Rkia Dardari and Renaud Léguillette

    Citation: BMC Molecular Biology 2011 12:5

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  2. Content type: Research article

    The Rasd1 protein is a dexamethasone induced monomeric Ras-like G protein that oscillates in the suprachiasmatic nucleus (SCN). Previous studies have shown that Rasd1 modulates multiple signaling cascades. How...

    Authors: Jen Jen Tan, Shufen Angeline Ong and Ken-Shiung Chen

    Citation: BMC Molecular Biology 2011 12:4

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  3. Content type: Methodology article

    With lower manufacturing cost, high spot density, and flexible probe design, genomic tiling microarrays are ideal for comprehensive transcriptome studies. Typically, transcriptome profiling using microarrays i...

    Authors: Wen-Han Yu, Hedda Høvik, Ingar Olsen and Tsute Chen

    Citation: BMC Molecular Biology 2011 12:3

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  4. Content type: Methodology article

    Brown algae of the genus Ectocarpus exhibit high levels of genetic diversity and variability in morphological and physiological characteristics. With the establishment of E. siliculosus as a model and the availab...

    Authors: Simon M Dittami, Caroline Proux, Sylvie Rousvoal, Akira F Peters, J Mark Cock, Jean-Yves Coppée, Catherine Boyen and Thierry Tonon

    Citation: BMC Molecular Biology 2011 12:2

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  5. Content type: Research article

    TEAD1 (TEA domain family member 1) is constitutively expressed in cardiac and skeletal muscles. It acts as a key molecule of muscle development, and trans-activates multiple target genes involved in cell prolifer...

    Authors: Haifang Qiu, Fengli Wang, Chuxin Liu, Xuewen Xu and Bang Liu

    Citation: BMC Molecular Biology 2011 12:1

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  6. Content type: Research article

    The synchrony of an organism with both its external and internal environment is critical to well-being and survival. As a result, organisms display daily cycles of physiology and behavior termed circadian rhyt...

    Authors: Kallur S Naidu, Louis W Morgan and Michael J Bailey

    Citation: BMC Molecular Biology 2010 11:104

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  7. Content type: Methodology article

    The importance of appropriate normalization controls in quantitative real-time polymerase chain reaction (qPCR) experiments has become more apparent as the number of biological studies using this methodology h...

    Authors: Meesbah Jiwaji, Rónán Daly, Kshama Pansare, Pauline McLean, Jingli Yang, Walter Kolch and Andrew R Pitt

    Citation: BMC Molecular Biology 2010 11:103

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  8. Content type: Research article

    The ubiquitous family of DnaN sliding processivity clamp proteins plays essential roles in DNA replication, DNA repair, and cell cycle progression, in part by managing the actions of the different proteins inv...

    Authors: Mark D Sutton, Jill M Duzen and Sarah K Scouten Ponticelli

    Citation: BMC Molecular Biology 2010 11:102

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  9. Content type: Research article

    The CTCF insulator protein is a highly conserved zinc finger protein that has been implicated in many aspects of gene regulation and nuclear organization. The protein has been hypothesized to organize the huma...

    Authors: Melissa J MacPherson and Paul D Sadowski

    Citation: BMC Molecular Biology 2010 11:101

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  10. Content type: Research article

    Gastrointestinal stromal tumors (GIST) represent the most common mesenchymal tumors of the gastrointestinal tract. About 85% carry an activating mutation in the KIT or PDGFRA gene. Approximately 10% of GIST are s...

    Authors: Jana Fassunke, Marie-Christine Blum, Hans-Ulrich Schildhaus, Marc Zapatka, Benedikt Brors, Helen Künstlinger, Reinhard Büttner, Eva Wardelmann and Sabine Merkelbach-Bruse

    Citation: BMC Molecular Biology 2010 11:100

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  11. Content type: Research article

    Postgenomic transcriptome analyses have identified large numbers of noncoding (nc)RNAs in mammalian cells. However, the biological function of long ncRNAs in mammalian cells remains largely unknown. Our recent...

    Authors: Kazuma Aoki, Akira Harashima, Miho Sano, Takahide Yokoi, Shuji Nakamura, Masayoshi Kibata and Tetsuro Hirose

    Citation: BMC Molecular Biology 2010 11:99

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  12. Content type: Research article

    Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Am...

    Authors: Fei Han, Zhijian Wang, Fengrui Wu, Zhihao Liu, Baofeng Huang and Deshou Wang

    Citation: BMC Molecular Biology 2010 11:98

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  13. Content type: Research article

    Alternative processing of α-thyroid hormone receptor (TRα, NR1A1) mRNAs gives rise to two functionally antagonistic nuclear receptors: TRα1, the α-type receptor, and TRα2, a non-hormone binding variant that is...

    Authors: Brandon C Rindfleisch, M Scott Brown, John L VandeBerg and Stephen H Munroe

    Citation: BMC Molecular Biology 2010 11:97

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  14. Content type: Research article

    Despite extensive efforts devoted to predicting protein-coding genes in genome sequences, many bona fide genes have not been found and many existing gene models are not accurate in all sequenced eukaryote genomes...

    Authors: Matthew J Nesbitt, Donald G Moerman and Nansheng Chen

    Citation: BMC Molecular Biology 2010 11:96

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  15. Content type: Research article

    Neurotrophins and their receptors are key molecules in the regulation of neuronal differentiation and survival. They mediate the survival of neurons during development and adulthood and are implicated in synap...

    Authors: Monica Guidi, Margarita Muiños-Gimeno, Birgit Kagerbauer, Eulàlia Martí, Xavier Estivill and Yolanda Espinosa-Parrilla

    Citation: BMC Molecular Biology 2010 11:95

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  16. Content type: Research article

    Amplification of single-stranded DNA circles has wide utility for a variety of applications. The two-primer ramified rolling circle amplification (RAM) reaction provides exponential DNA amplification under iso...

    Authors: Thomas P Beals, James H Smith, Raymond M Nietupski and David J Lane

    Citation: BMC Molecular Biology 2010 11:94

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  17. Content type: Research article

    RNA interference (RNAi) emerges as a powerful tool to induce loss-of-function phenotypes. In the context of the brain, gene manipulation is best targeted to specific subsets of cells in order to achieve a phys...

    Authors: Beihui Liu, Haibo Xu, Julian FR Paton and Sergey Kasparov

    Citation: BMC Molecular Biology 2010 11:93

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  18. Content type: Research article

    In Saccharomyces cerevisiae, the immunosuppressant rapamycin engenders a profound modification in the transcriptional profile leading to growth arrest. Mutants devoid of Rrd1, a protein possessing in vitro peptid...

    Authors: Nathalie Jouvet, Jeremie Poschmann, Julie Douville, Lisa Bulet and Dindial Ramotar

    Citation: BMC Molecular Biology 2010 11:92

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  19. Content type: Research article

    Activated leukocyte cell adhesion molecule (ALCAM/CD166) is expressed by hematopoietic stem cells. However, its role in hematopoietic differentiation has not previously been defined.

    Authors: Fang Tan, Samit Ghosh, Flaubert Mbeunkui, Robert Thomas, Joshua A Weiner and Solomon F Ofori-Acquah

    Citation: BMC Molecular Biology 2010 11:91

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  20. Content type: Research article

    Reliable reference genes are a vital prerequisite for any functional study employing quantitative real-time RT-PCR (RT-qPCR) for analyzing gene expression. Yet a proper selection and assessment of the chosen r...

    Authors: Marten B Maeß, Stefanie Sendelbach and Stefan Lorkowski

    Citation: BMC Molecular Biology 2010 11:90

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  21. Content type: Research article

    The promyelocytic leukemia (PML) protein participates in a number of cellular processes, including transcription regulation, apoptosis, differentiation, virus defense and genome maintenance. This protein is st...

    Authors: Åsne Jul-Larsen, Amra Grudic, Rolf Bjerkvig and Stig O Bøe

    Citation: BMC Molecular Biology 2010 11:89

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  22. Content type: Research article

    Geranyl pyrophosphate (GPP) and p-hydroxybenzoate (PHB) are the basic precursors involved in shikonins biosynthesis. GPP is derived from mevalonate (MVA) and/or 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway(s...

    Authors: Ravi S Singh, Rishi K Gara, Pardeep K Bhardwaj, Anish Kaachra, Sonia Malik, Ravi Kumar, Madhu Sharma, Paramvir S Ahuja and Sanjay Kumar

    Citation: BMC Molecular Biology 2010 11:88

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  23. Content type: Research article

    Most type II restriction-modification (RM) systems have two independent enzymes that act on the same DNA sequence: a modification methyltransferase that protects target sites, and a restriction endonuclease th...

    Authors: Meenakshi K Kaw and Robert M Blumenthal

    Citation: BMC Molecular Biology 2010 11:87

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  24. Content type: Methodology article

    Stalk-eyed flies of the family Diopsidae have proven to be an excellent model organism for studying the evolution of ornamental sexual traits. In diopsid flies the eyes and antennae are borne at the end of lat...

    Authors: Ian A Warren, Kevin Fowler and Hazel Smith

    Citation: BMC Molecular Biology 2010 11:86

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  25. Content type: Research article

    Eukaryotic transcription activators normally consist of a sequence-specific DNA-binding domain (DBD) and a transcription activation domain (AD). While many sequence patterns and motifs have been defined for DB...

    Authors: Chen-Huan Lin, Grace Lin, Chia-Pei Chang and Chien-Chia Wang

    Citation: BMC Molecular Biology 2010 11:85

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  26. Content type: Research article

    Dengue viruses (DENV) are one of the most important viral diseases in the world with approximately 100 million infections and 200,000 deaths each year. The current lack of an approved tetravalent vaccine and i...

    Authors: James R Carter, James H Keith, Pradip V Barde, Tresa S Fraser and Malcolm J Fraser Jr

    Citation: BMC Molecular Biology 2010 11:84

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  27. Content type: Research article

    Human cells depend critically on the signal recognition particle (SRP) for the sorting and delivery of their proteins. The SRP is a ribonucleoprotein complex which binds to signal sequences of secretory polype...

    Authors: Elena Iakhiaeva, Alexei Iakhiaev and Christian Zwieb

    Citation: BMC Molecular Biology 2010 11:83

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  28. Content type: Research article

    Purα is an evolutionarily conserved cellular protein participating in processes of DNA replication, transcription, and RNA transport; all involving binding to nucleic acids and altering conformation and physic...

    Authors: Margaret J Wortman, Laura K Hanson, Luis Martínez-Sobrido, Ann E Campbell, Jonas A Nance, Adolfo García-Sastre and Edward M Johnson

    Citation: BMC Molecular Biology 2010 11:81

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  29. Content type: Research article

    In male Drosophila melanogaster, the male specific lethal (MSL) complex is somehow responsible for a two-fold increase in transcription of most X-linked genes, which are enriched for histone H4 acetylated at lysi...

    Authors: Anja H Schiemann, Fang Li, Vikki M Weake, Esther J Belikoff, Kent C Klemmer, Stanley A Moore and Maxwell J Scott

    Citation: BMC Molecular Biology 2010 11:80

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  30. Content type: Research article

    Carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is a transmembrane protein with multiple functions in different cell types. CEACAM1 expression is frequently mis-regulated in cancer, with down-regul...

    Authors: Marieta Gencheva, Charng-Jui Chen, Tung Nguyen and John E Shively

    Citation: BMC Molecular Biology 2010 11:79

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  31. Content type: Research article

    Protein tyrosine phosphatases (PTPs) are important cell signaling regulators with major pathological implications. LYP (also known as PTPN22) is an intracellular enzyme initially found to be predominately expr...

    Authors: Shaofeng Wang, Hongbo Dong, Jiayu Han, Wanting T Ho, Xueqi Fu and Zhizhuang J Zhao

    Citation: BMC Molecular Biology 2010 11:78

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  32. Content type: Methodology article

    Combinatorial RNA interference (co-RNAi) is a valuable tool for highly effective gene suppression of single and multiple-genes targets, and can be used to prevent the escape of mutation-prone transcripts. Ther...

    Authors: Luke S Lambeth, Nick J Van Hateren, Stuart A Wilson and Venugopal Nair

    Citation: BMC Molecular Biology 2010 11:77

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  33. Content type: Research article

    Quantitative real-time reverse transcriptase PCR (RT-qPCR) has been widely used for quantification of mRNA as a way to determine key genes involved in different biological processes. For accurate gene quantifi...

    Authors: Guang-Mao Shen, Hong-Bo Jiang, Xiao-Na Wang and Jin-Jun Wang

    Citation: BMC Molecular Biology 2010 11:76

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  34. Content type: Research article

    Quantitative real-time PCR (qPCR) has been the method of choice for the quantification of mRNA. Due to the various artifactual factors that may affect the accuracy of qPCR, internal reference genes are most of...

    Authors: Alvin N Setiawan and P Mark Lokman

    Citation: BMC Molecular Biology 2010 11:75

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  35. Content type: Editorial

    The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combin...

    Authors: Stephen A Bustin, Jean-François Beaulieu, Jim Huggett, Rolf Jaggi, Frederick SB Kibenge, Pål A Olsvik, Louis C Penning and Stefan Toegel

    Citation: BMC Molecular Biology 2010 11:74

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  36. Content type: Research article

    Eucalyptus globulus and its hybrids are very important for the cellulose and paper industry mainly due to their low lignin content and frost resistance. However, rooting of cuttings of this species is recalcitran...

    Authors: Márcia R de Almeida, Carolina M Ruedell, Felipe K Ricachenevsky, Raul A Sperotto, Giancarlo Pasquali and Arthur G Fett-Neto

    Citation: BMC Molecular Biology 2010 11:73

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  37. Content type: Research article

    Based on its activities in vitro, the mammalian mitochondrial transcription termination factor mTERF has been proposed to regulate mitochondrial transcription by favouring termination at its high-affinity binding...

    Authors: Anne K Hyvärinen, Mona K Kumanto, Sanna K Marjavaara and Howard T Jacobs

    Citation: BMC Molecular Biology 2010 11:72

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  38. Content type: Research article

    Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received l...

    Authors: Ya-Nan Gong, Wei-Wei Li, Jiang-Ling Sun, Fei Ren, Lin He, Hui Jiang and Qun Wang

    Citation: BMC Molecular Biology 2010 11:71

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  39. Content type: Research article

    During the last two decades, DNA sequencing has led to the identification of numerous genes in key species; however, in most cases, their functions are still unknown. In this situation, reverse genetics is the...

    Authors: Tomoko Ishikawa, Yasuhiro Kamei, Shinji Otozai, Jinhyong Kim, Ayuko Sato, Yoshikazu Kuwahara, Minoru Tanaka, Tomonori Deguchi, Hidenori Inohara, Tohru Tsujimura and Takeshi Todo

    Citation: BMC Molecular Biology 2010 11:70

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  40. Content type: Research article

    Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integr...

    Authors: Natali Ozber, Ibrahim Baris, Gulnaz Tatlici, Ibrahim Gur, Seda Kilinc, Evrim B Unal and Ibrahim H Kavakli

    Citation: BMC Molecular Biology 2010 11:69

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  41. Content type: Research article

    The ETS transcription factor Elf5 (also known as ESE-2) is highly expressed in the mammary gland and plays an important role in its development and differentiation. Indeed studies in mice have illustrated an e...

    Authors: Rosalba Escamilla-Hernandez, Rumela Chakrabarti, Rose-Anne Romano, Kirsten Smalley, Qianqian Zhu, William Lai, Marc S Halfon, Michael J Buck and Satrajit Sinha

    Citation: BMC Molecular Biology 2010 11:68

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  42. Content type: Research article

    The c-myb proto-oncogene is the founding member of a family of transcription factors involved principally in haematopoiesis, in diverse organisms, from zebrafish to mammals. Its deregulation has been implicated i...

    Authors: Audrey Guillon-Munos, Ginette Dambrine, Nicolas Richerioux, Damien Coupeau, Benoît Muylkens and Denis Rasschaert

    Citation: BMC Molecular Biology 2010 11:67

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  43. Content type: Research article

    Postnatal expansion of the pancreatic β-cell mass is required to maintain glucose homeostasis immediately after birth. This β-cell expansion is regulated by multiple growth factors, including glucose, insulin,...

    Authors: Haijuan Wang, Katarina Gambosova, Zachary A Cooper, Michael P Holloway, Andrea Kassai, Denisse Izquierdo, Kelly Cleveland, Charlotte M Boney and Rachel A Altura

    Citation: BMC Molecular Biology 2010 11:66

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  44. Content type: Research article

    In insects, hemocyanin superfamily proteins accumulate apparently to serve as sources of amino acids during metamorphosis, reproduction and development. Storage hexamerins are important members of the hemocyan...

    Authors: Bin Tang, Shigui Wang and Fan Zhang

    Citation: BMC Molecular Biology 2010 11:65

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  45. Content type: Research article

    The L1 cell adhesion molecule (L1CAM) was originally identified as a neural adhesion molecule involved in axon guidance. In many human epithelial carcinomas L1CAM is overexpressed and thereby augments cell mot...

    Authors: Marco Pfeifer, Uwe Schirmer, Claudia Geismann, Heiner Schäfer, Susanne Sebens and Peter Altevogt

    Citation: BMC Molecular Biology 2010 11:64

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  46. Content type: Research article

    Smith-Magenis Syndrome is a contiguous gene syndrome in which the dosage sensitive gene has been identified: the Retinoic Acid Induced 1 (RAI1). Little is known about the function of human RAI1.

    Authors: Paulina Carmona-Mora, Carolina A Encina, Cesar P Canales, Lei Cao, Jessica Molina, Pamela Kairath, Juan I Young and Katherina Walz

    Citation: BMC Molecular Biology 2010 11:63

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  47. Content type: Research article

    The mini-chromosome maintenance protein (MCM) complex is an essential replicative helicase for DNA replication in Archaea and Eukaryotes. While the eukaryotic complex consists of six homologous proteins (MCM2-...

    Authors: Aaron S Brewster, Ian M Slaymaker, Samir A Afif and Xiaojiang S Chen

    Citation: BMC Molecular Biology 2010 11:62

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  48. Content type: Methodology article

    RT-qPCR analysis is a widely used method for the analysis of mRNA expression throughout the field of mesenchymal stromal cell (MSC) research. Comparison between MSC studies, both in vitro and in vivo, are challen...

    Authors: Kevin M Curtis, Lourdes A Gomez, Carmen Rios, Elisa Garbayo, Ami P Raval, Miguel A Perez-Pinzon and Paul C Schiller

    Citation: BMC Molecular Biology 2010 11:61

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  49. Content type: Research article

    Circadian rhythms have a profound effect on human health. Their disruption can lead to serious pathologies, such as cancer and obesity. Gene expression studies in these pathologies are often studied in differe...

    Authors: Rok Kosir, Jure Acimovic, Marko Golicnik, Martina Perse, Gregor Majdic, Martina Fink and Damjana Rozman

    Citation: BMC Molecular Biology 2010 11:60

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