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  1. Preparation of RNA free from DNA is a critical step before performing RT-PCR assay. Total RNA isolated from several sources, including those obtained from Saccharomyces cerevisiae, using routine methodologies are...

    Authors: Eduardo M Del Aguila, Marcio B Dutra, Joab T Silva and Vânia MF Paschoalin

    Citation: BMC Molecular Biology 2005 6:9

    Content type: Research article

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  2. The promoter of the keratin 18 (K18) gene is 5- to 10-fold more active in tumorigenic (T-type) cell clones derived from the SW613-S human colon carcinoma cell line than in non-tumorigenic (NT-type) clones. We ...

    Authors: Cécile Delouis, Philippe Prochasson, Madeleine Laithier and Olivier Brison

    Citation: BMC Molecular Biology 2005 6:8

    Content type: Research article

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  3. The current investigation was undertaken to determine key steps differentiating G:T and G:A repair at the H-ras oncogenic hot spot within the nuclear environment because of the large difference in repair efficien...

    Authors: Michael Edelbrock, Huiling He, Allen Schroering, Martha Fernstrom, Sangeetha Bathala and Kandace J Williams

    Citation: BMC Molecular Biology 2005 6:6

    Content type: Research article

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  4. Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with ...

    Authors: Ashwini S Kucknoor, Vasanthakrishna Mundodi and JF Alderete

    Citation: BMC Molecular Biology 2005 6:5

    Content type: Research article

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  5. The parasitic protozoa belonging to Leishmania (L.) donovani complex possess abundant, developmentally regulated cathepsin L-like cysteine proteases. Previously, we have reported the isolation of cysteine proteas...

    Authors: Vasanthakrishna Mundodi, Ashwini S Kucknoor and Lashitew Gedamu

    Citation: BMC Molecular Biology 2005 6:3

    Content type: Research article

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  6. The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase ge...

    Authors: Gerald Thiel, Jude Al Sarraj and Luisa Stefano

    Citation: BMC Molecular Biology 2005 6:2

    Content type: Research article

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  7. Coupling of alternative splicing with nonsense-mediated mRNA decay (NMD) may regulate gene expression. We report here the identification of a nonsense alternative transcript of the fumarylacetoacetate hydrolase (

    Authors: Natacha Dreumont, Antonella Maresca, Jean-François Boisclair-Lachance, Anne Bergeron and Robert M Tanguay

    Citation: BMC Molecular Biology 2005 6:1

    Content type: Research article

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  8. An Escherichia coli strain in which RecBCD has been genetically replaced by the bacteriophage λ Red system engages in efficient recombination between its chromosome and linear double-stranded DNA species sharing ...

    Authors: Anthony R Poteete, Anita C Fenton and Ashwini Nadkarni

    Citation: BMC Molecular Biology 2004 5:22

    Content type: Research article

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  9. DNA polymerases α and δ play essential roles in the replication of chromosomal DNA in eukaryotic cells. DNA polymerase α (Pol α)-primase is required to prime synthesis of the leading strand and each Okazaki fr...

    Authors: Fiona C Gray, J Richard G Pohler, Emma Warbrick and Stuart A MacNeill

    Citation: BMC Molecular Biology 2004 5:21

    Content type: Research article

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  10. Methyl-DNA binding proteins help to translate epigenetic information encoded by DNA methylation into covalent histone modifications. MBD2/3 is the only candidate gene in the Drosophila genome with extended homolo...

    Authors: Joachim Marhold, Alexander Brehm and Katja Kramer

    Citation: BMC Molecular Biology 2004 5:20

    Content type: Research article

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  11. The expression of glucocorticoid-receptor (GR) seems to be a key mechanism in the regulation of glucocorticoid (GC) sensitivity and is potentially involved in cases of GC resistance or hypersensitivity. The ai...

    Authors: Murilo R Melo, Cláudia DC Faria, Keli C Melo, Nancy A Rebouças and Carlos A Longui

    Citation: BMC Molecular Biology 2004 5:19

    Content type: Methodology article

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  12. The circadian rhythm of about 24 hours is a fundamental physiological function observed in almost all organisms from prokaryotes to humans. Identification of clock genes has allowed us to study the molecular b...

    Authors: Takuro Yamamoto, Yasukazu Nakahata, Haruhiko Soma, Makoto Akashi, Takayoshi Mamine and Toru Takumi

    Citation: BMC Molecular Biology 2004 5:18

    Content type: Research article

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  13. Foamy virus Bel1/Tas trans-activators act as key regulators of gene expression and directly bind to Bel1 response elements (BRE) in both the internal and the 5'LTR promoters leading to strong transcriptional tran...

    Authors: Helmut Bannert, Walter Muranyi, Vasily V Ogryzko, Yoshihiro Nakatani and Rolf M Flügel

    Citation: BMC Molecular Biology 2004 5:16

    Content type: Research article

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  14. Hepatic expression of several gene products involved in glucose metabolism, including phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6Pase) and insulin-like growth factor binding protein-1 ...

    Authors: David Finlay, Satish Patel, Lorna M Dickson, Natalia Shpiro, Rodolfo Marquez, Chris J Rhodes and Calum Sutherland

    Citation: BMC Molecular Biology 2004 5:15

    Content type: Research article

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  15. The periodic pattern of DNA in exons is a known phenomenon. It was suggested that one of the initial causes of periodicity could be the universal (RNY) n pattern (R = A or G,...

    Authors: Stephen T Eskesen, Frank N Eskesen, Brian Kinghorn and Anatoly Ruvinsky

    Citation: BMC Molecular Biology 2004 5:12

    Content type: Research article

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  16. p68 (Ddx5) and p72 (Ddx17) are highly related members of the DEAD box family and are established RNA helicases. They have been implicated in growth regulation and have been shown to be involved in both pre-mRN...

    Authors: Brian J Wilson, Gaynor J Bates, Samantha M Nicol, David J Gregory, Neil D Perkins and Frances V Fuller-Pace

    Citation: BMC Molecular Biology 2004 5:11

    Content type: Research article

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  17. Most of the existing RNA structure prediction programs fold a completely synthesized RNA molecule. However, within the cell, RNA molecules emerge sequentially during the directed process of transcription. Dedi...

    Authors: Irmtraud M Meyer and István Miklós

    Citation: BMC Molecular Biology 2004 5:10

    Content type: Research article

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  18. Incorporation of exon 11 of the insulin receptor gene is both developmentally and hormonally-regulated. Previously, we have shown the presence of enhancer and silencer elements that modulate the incorporation ...

    Authors: Nicholas JG Webster, Lui-Guojing Evans, Matt Caples, Laura Erker and Shern L Chew

    Citation: BMC Molecular Biology 2004 5:7

    Content type: Research article

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  19. Although the complete genome sequences of a large number of organisms have been determined, the exact proteomes need to be characterized. More specifically, the extent to which post-translational processes suc...

    Authors: Rajinder Kumar, Alla Musiyenko, Anja Oldenburg, Brian Adams and Sailen Barik

    Citation: BMC Molecular Biology 2004 5:6

    Content type: Research article

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  20. Exposure of Leishmania promastigotes to the temperature of their mammalian hosts results in the induction of a typical heat shock response. It has been suggested that heat shock proteins play an important role in...

    Authors: Ruth Larreta, Manuel Soto, Luis Quijada, Cristina Folgueira, Daniel R Abanades, Carlos Alonso and Jose M Requena

    Citation: BMC Molecular Biology 2004 5:3

    Content type: Research article

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  21. Despite the importance of glucocorticoids in suppressing immune and inflammatory responses, their role in enhancing host immune and defense response against invading bacteria is poorly understood. Toll-like re...

    Authors: Akihiro Sakai, Jiahuai Han, Andrew CB Cato, Shizuo Akira and Jian-Dong Li

    Citation: BMC Molecular Biology 2004 5:2

    Content type: Research article

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  22. The mammalian protein kinase TLK1 is a homologue of Tousled, a gene involved in flower development in Arabidopsis thaliana. The function of TLK1 is not well known, although knockout of the gene in Drosophila, or ...

    Authors: Gulshan Sunavala-Dossabhoy, Marcie Fowler and Arrigo De Benedetti

    Citation: BMC Molecular Biology 2004 5:1

    Content type: Research article

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  23. The Wnt signaling pathway plays critical roles in cell proliferation and cell fate determination at many stages of development. A critical downstream target of Wnt signaling is the cytosolic β-catenin, which i...

    Authors: Feng Cong, Jianxuan Zhang, William Pao, Pengbo Zhou and Harold Varmus

    Citation: BMC Molecular Biology 2003 4:10

    Content type: Research article

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  24. Pifithrin-α is a small molecule inhibitor of p53 transcriptional activity. It has been proposed that the use of pifithrin-α in conjunction with chemotherapeutic and radiation therapies for cancer will reduce t...

    Authors: Sonia Rocha, Kirsteen J Campbell, Kevin C Roche and Neil D Perkins

    Citation: BMC Molecular Biology 2003 4:9

    Content type: Research article

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  25. In mammals, epigenetic information is established and maintained via the postreplicative methylation of cytosine residues by the DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Dnmt1 is required for maintenan...

    Authors: Jean B Margot, Ann E Ehrenhofer-Murray and Heinrich Leonhardt

    Citation: BMC Molecular Biology 2003 4:7

    Content type: Research article

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  26. The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos.

    Authors: Sebastian Schuck, Ana Soloaga, Gerhard Schratt, J Simon C Arthur and Alfred Nordheim

    Citation: BMC Molecular Biology 2003 4:6

    Content type: Research article

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  27. After intravenous delivery of the adenoviral vector into rats or mice, 95–99% of the encoded protein is produced in the hepatocytes. We observed, as have others, that the early expression levels of the vector ...

    Authors: Pierre Lefesvre, Joline Attema, Angelique Lemckert, Menzo Havenga and Dirk van Bekkum

    Citation: BMC Molecular Biology 2003 4:4

    Content type: Research article

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  28. Expansion of an unstable (CGG)n repeat to over 200 triplets within the promoter region of the human FMR1 gene leads to extensive local methylation and transcription silencing, resulting in the loss of FMRP protei...

    Authors: Simon P Chandler, Pushpa Kansagra and Mark C Hirst

    Citation: BMC Molecular Biology 2003 4:3

    Content type: Research article

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  29. Termination of protein synthesis in eukaryotes involves at least two polypeptide release factors (eRFs) – eRF1 and eRF3. The highly conserved translation termination factor eRF1 in Saccharomyces cerevisiae is enc...

    Authors: Svetlana E Moskalenko, Svetlana V Chabelskaya, Sergei G Inge-Vechtomov, Michel Philippe and Galina A Zhouravleva

    Citation: BMC Molecular Biology 2003 4:2

    Content type: Research article

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  30. The phage protein pairs, RecE/RecT from Rac or Redα/Redβ from λ, initiate efficient double strand break repair (DSBR) in Escherichia coli that has proven very useful for DNA engineering. These phage pairs initiat...

    Authors: Youming Zhang, Joep PP Muyrers, Jeanette Rientjes and A Francis Stewart

    Citation: BMC Molecular Biology 2003 4:1

    Content type: Research article

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  31. NF-κB is implicated in gene regulation involved in neuronal survival, inflammmatory response and cancer. There are relatively few neuronal target genes of NF-κB characterized.

    Authors: Barbara Kaltschmidt, Ralf A Linker, Jinbo Deng and Christian Kaltschmidt

    Citation: BMC Molecular Biology 2002 3:16

    Content type: Research article

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  32. Secretion of recombinant proteins in yeast can be affected by their improper folding in the endoplasmic reticulum and subsequent elimination of the misfolded molecules via the endoplasmic reticulum associated pro...

    Authors: Michael O Agaphonov, Nina V Romanova, Polina M Trushkina, Vladimir N Smirnov and Michael D Ter-Avanesyan

    Citation: BMC Molecular Biology 2002 3:15

    Content type: Research article

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  33. Tetracycline-regulated systems have been used to control the expression of heterologous genes in such diverse organisms as yeast, plants, flies and mice. Adaptation of this prokaryotic regulatory system avoids...

    Authors: Paraic A Kenny, Tariq Enver and Alan Ashworth

    Citation: BMC Molecular Biology 2002 3:13

    Content type: Methodology article

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  34. The tumor suppressor gene PTEN has been found mutated in many types of advanced tumors. When introduced into tumor cells that lack the wild-type allele of the gene, exogenous PTEN was able to suppress their ab...

    Authors: Ray-Chang Wu, Martina Blumenthal, Xinwei Li and Axel H Schönthal

    Citation: BMC Molecular Biology 2002 3:11

    Content type: Research article

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  35. Cyclic AMP-dependent protein kinase (PKA) plays a central role in regulation of energy metabolism. Upon stimulation of testicular Sertoli cells by follicle stimulating hormone (FSH), glycolysis is activated to...

    Authors: Maria Krudtaa Dahle, Kjetil Taskén and Kristin Austlid Taskén

    Citation: BMC Molecular Biology 2002 3:10

    Content type: Research article

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