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  1. Human eosinophil-derived neurotoxin (edn) and eosinophil cationic protein (ecp) are members of a subfamily of primate ribonuclease (rnase) genes. Although they are generated by gene duplication event, distinct ed...

    Authors: Hsiu-Yu Wang, Hao-Teng Chang, Tun-Wen Pai, Chung-I Wu, Yuan-Hung Lee, Yen-Hsin Chang, Hsiu-Ling Tai, Chuan-Yi Tang, Wei-Yao Chou and Margaret Dah-Tsyr Chang

    Citation: BMC Molecular Biology 2007 8:89

    Content type: Research article

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  2. Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active ...

    Authors: Hongshan Chen, Wei Mo, Huabo Su, Yanling Zhang and Houyan Song

    Citation: BMC Molecular Biology 2007 8:88

    Content type: Research article

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  3. The 2,2,7-trimethylguanosine (TMG) cap structure is an important functional characteristic of ncRNAs with critical cellular roles, such as some snRNAs. Here we used immunoprecipitation with both K121 and R1131...

    Authors: Dong Jia, Lun Cai, Housheng He, Geir Skogerbø, Tiantian Li, Muhammad Nauman Aftab and Runsheng Chen

    Citation: BMC Molecular Biology 2007 8:86

    Content type: Research article

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  4. Genetic experiments have clarified that p63 is a key transcription factor governing the establishment and maintenance of multilayered epithelia. Key to our understanding of p63 strategy is the identification o...

    Authors: Serena Borrelli, Barbara Testoni, Maurizio Callari, Daniela Alotto, Carlotta Castagnoli, Rose-Anne Romano, Satrajit Sinha, Alessandra M Viganò and Roberto Mantovani

    Citation: BMC Molecular Biology 2007 8:85

    Content type: Research article

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  5. The homeodomain-containing transcription factor PITX3 was shown to be essential for normal eye development in vertebrates. Human patients with point mutations in PITX3 demonstrate congenital cataracts along with ...

    Authors: Satoru Sakazume, Elena Sorokina, Yoshiki Iwamoto and Elena V Semina

    Citation: BMC Molecular Biology 2007 8:84

    Content type: Research article

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  6. Alternative splicing is a widespread mechanism of gene expression regulation. Previous analyses based on conventional RT-PCR reported the presence of an unspliced c-fos transcript in several mammalian systems. Co...

    Authors: Juan Jurado, Carlos A Fuentes-Almagro, María J Prieto-Álamo and Carmen Pueyo

    Citation: BMC Molecular Biology 2007 8:83

    Content type: Research article

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  7. The Entamoeba histolytica EhrabB gene encodes for a Rab GTPase involved in phagocytosis. It is located at a virulence locus where the Ehcp112 gene is in the complementary strand at 332 bp of EhrabB start codon, s...

    Authors: Mónica Romero-Díaz, Consuelo Gómez, Israel López-Reyes, Máximo B Martínez, Esther Orozco and Mario A Rodríguez

    Citation: BMC Molecular Biology 2007 8:82

    Content type: Research article

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  8. Genome integrity is constantly challenged and requires the coordinated recruitment of multiple enzyme activities to ensure efficient repair of DNA lesions. We investigated the dynamics of XRCC1 and PCNA that a...

    Authors: Oliver Mortusewicz and Heinrich Leonhardt

    Citation: BMC Molecular Biology 2007 8:81

    Content type: Research article

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  9. The Gateway recombinatorial cloning system allows easy and rapid joining of DNA fragments. Here we report the construction and evaluation of three different Gram-positive vectors that can be used with the Mult...

    Authors: Tania M Perehinec, Saara NA Qazi, Sanyasi R Gaddipati, Vyvyan Salisbury, Catherine ED Rees and Philip J Hill

    Citation: BMC Molecular Biology 2007 8:80

    Content type: Methodology article

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  10. MicroRNAs (miRNAs) are one of the most abundant groups of regulatory genes in multicellular organisms, playing important roles in many fundamental cellular processes. More than four hundred miRNAs have been id...

    Authors: Edyta Koscianska, Vesselin Baev, Konstantinia Skreka, Katerina Oikonomaki, Ventsislav Rusinov, Martin Tabler and Kriton Kalantidis

    Citation: BMC Molecular Biology 2007 8:79

    Content type: Methodology article

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  11. We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inac...

    Authors: Raffaella Di Lisi, Anne Picard, Simonetta Ausoni and Stefano Schiaffino

    Citation: BMC Molecular Biology 2007 8:78

    Content type: Research article

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  12. Most molecular biology experiments, and the techniques associated with this field of study, involve a great deal of engineering in the form of molecular cloning. Like all forms of engineering, perfect informat...

    Authors: Alexandre Angers-Loustau, Jeffrey Rainy and Kirmo Wartiovaara

    Citation: BMC Molecular Biology 2007 8:77

    Content type: Software

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  13. Huntington's disease, spinal and bulbar muscular atrophy, and spinocerebellar ataxia 17 (SCA17) are caused by expansions in the polyglutamine (polyQ) repeats in Huntingtin protein (Htt), androgen receptor prot...

    Authors: Justin R Prigge and Edward E Schmidt

    Citation: BMC Molecular Biology 2007 8:76

    Content type: Research article

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  14. The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the transformation of myelomonocytic cells by avian myeloblastosis virus (AMV). v-Myb is thought to exert its biologic...

    Authors: Holger Gundelach, Daniel Braas and Karl-Heinz Klempnauer

    Citation: BMC Molecular Biology 2007 8:75

    Content type: Research article

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  15. Cone degeneration is the hallmark of the inherited retinal disease retinitis pigmentosa. We have previously identified a trophic factor "Rod-derived Cone Viability Factor (RdCVF) that is secreted by rods and p...

    Authors: Frédéric Chalmel, Thierry Léveillard, Céline Jaillard, Aurélie Lardenois, Naomi Berdugo, Emmanuelle Morel, Patrice Koehl, George Lambrou, Arne Holmgren, José A Sahel and Olivier Poch

    Citation: BMC Molecular Biology 2007 8:74

    Content type: Research article

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  16. The tight organisation of eukaryotic genomes as chromatin hinders the interaction of many DNA-binding regulators. The local accessibility of DNA is regulated by many chromatin modifying enzymes, among them the...

    Authors: Roger Ferreira, Anton Eberharter, Tiziana Bonaldi, Mariacristina Chioda, Axel Imhof and Peter B Becker

    Citation: BMC Molecular Biology 2007 8:73

    Content type: Research article

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  17. Tumors expressing a transforming growth factor-beta type I receptor (TβRI) mutant with sequence deletions in a nine-alanine (9A) stretch of the signal peptide are reported to be highly associated with disease ...

    Authors: Kuo-Li Chen, Wan-Hsin Liu, Yi-Yuan Yang, Sy-Jye C Leu and Neng-Yao Shih

    Citation: BMC Molecular Biology 2007 8:72

    Content type: Research article

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  18. The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature termination codons (PTCs). In yeast Saccharomyces cerevisiae, the activity of the NMD pathway depends on...

    Authors: Svetlana Chabelskaya, Valentina Gryzina, Svetlana Moskalenko, Catherine Le Goff and Galina Zhouravleva

    Citation: BMC Molecular Biology 2007 8:71

    Content type: Research article

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  19. Peroxisome proliferator-activated receptor delta (PPARδ) is a member of the nuclear receptor superfamily. Numerous studies have aimed at unravelling the physiological role of PPARδ as a transcriptional regulat...

    Authors: Kerstin Lundell, Petra Thulin, Anders Hamsten and Ewa Ehrenborg

    Citation: BMC Molecular Biology 2007 8:70

    Content type: Research article

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  20. Enzymes involved in DNA metabolic events of the highly radioresistant bacterium Deinococcus radiodurans are currently examined to understand the mechanisms that protect and repair the Deinococcus radiodurans geno...

    Authors: Melanie Blasius, Rebecca Buob, Igor V Shevelev and Ulrich Hubscher

    Citation: BMC Molecular Biology 2007 8:69

    Content type: Research article

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  21. Recent analysis of immune-related genes within the sea urchin genome revealed a number of large gene families with vertebrate homologues, such as the Toll-like and NOD/NALP-like receptor families and C-type le...

    Authors: Katherine M Buckley and L Courtney Smith

    Citation: BMC Molecular Biology 2007 8:68

    Content type: Research article

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  22. Real-time quantitative PCR (qPCR) is a method for rapid and reliable quantification of mRNA transcription. Internal standards such as reference genes are used to normalise mRNA levels between different samples...

    Authors: Ann-Britt Nygard, Claus B Jørgensen, Susanna Cirera and Merete Fredholm

    Citation: BMC Molecular Biology 2007 8:67

    Content type: Methodology article

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  23. Nucleolin is a major component of the nucleolus, but is also found in other cell compartments. This protein is involved in various aspects of ribosome biogenesis from transcription regulation to the assembly o...

    Authors: Iva Ugrinova, Karine Monier, Corinne Ivaldi, Marc Thiry, Sébastien Storck, Fabien Mongelard and Philippe Bouvet

    Citation: BMC Molecular Biology 2007 8:66

    Content type: Research article

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  24. Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following t...

    Authors: Scott A Boden, Nadim Shadiac, Elise J Tucker, Peter Langridge and Jason A Able

    Citation: BMC Molecular Biology 2007 8:65

    Content type: Research article

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  25. Cytokines have essential roles on intercellular communications and are effective in using a variety of intracellular pathways. Among this multitude of signalling pathways, the NF-κB (nuclear factor kappaB) and...

    Authors: Sandrine Lafarge, Hind Hamzeh-Cognasse, Patricia Chavarin, Christian Genin, Olivier Garraud and Fabrice Cognasse

    Citation: BMC Molecular Biology 2007 8:64

    Content type: Research article

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  26. RNA interference (RNAi) is a regulatory mechanism conserved in higher eukaryotes. The RNAi pathway generates small interfering RNA (siRNA) or micro RNA (miRNA) from either long double stranded stretches of RNA...

    Authors: Zachary Klase, Prachee Kale, Rafael Winograd, Madhur V Gupta, Mohammad Heydarian, Reem Berro, Timothy McCaffrey and Fatah Kashanchi

    Citation: BMC Molecular Biology 2007 8:63

    Content type: Research article

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  27. Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RT-qPCR) is the most accurate measure of gene expression in biological systems. The comparison of different samples requires th...

    Authors: Lindsey J Maccoux, Dylan N Clements, Fiona Salway and Philip JR Day

    Citation: BMC Molecular Biology 2007 8:62

    Content type: Research article

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  28. Trinucleotide instability is a hallmark of degenerative neurological diseases like Huntington's disease, some forms of spinocerebellar ataxia and myotonic dystrophy type 1 (DM1). To investigate the effect of c...

    Authors: Walther JAA van den Broek, Derick G Wansink and Bé Wieringa

    Citation: BMC Molecular Biology 2007 8:61

    Content type: Research article

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  29. CD36 is a multiligand receptor involved in various metabolic pathways, including cellular uptake of long-chain fatty acids. Defect function or expression of CD36 can result in dyslipidemia or insulin resistanc...

    Authors: Louisa Cheung, Malin Andersen, Carolina Gustavsson, Jacob Odeberg, Leandro Fernández-Pérez, Gunnar Norstedt and Petra Tollet-Egnell

    Citation: BMC Molecular Biology 2007 8:60

    Content type: Research article

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  30. Japanese encephalitis virus (JEV) NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains. It is a key component of the viral RNA replicase comp...

    Authors: Yeon-Gu Kim, Ji-Seung Yoo, Jung-Hee Kim, Chan-Mi Kim and Jong-Won Oh

    Citation: BMC Molecular Biology 2007 8:59

    Content type: Research article

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  31. Antisense oligonucleotides (AOs) can interfere with exon recognition and intron removal during pre-mRNA processing, and induce excision of a targeted exon from the mature gene transcript. AOs have been used in vi...

    Authors: Abbie M Adams, Penny L Harding, Patrick L Iversen, Catherine Coleman, Sue Fletcher and Steve D Wilton

    Citation: BMC Molecular Biology 2007 8:57

    Content type: Research article

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  32. Gene transfer by electroporation (DNA electrotransfer) to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little ...

    Authors: Pernille Hojman, John R Zibert, Hanne Gissel, Jens Eriksen and Julie Gehl

    Citation: BMC Molecular Biology 2007 8:56

    Content type: Research article

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  33. Gene expression studies related to cancer diagnosis and treatment are becoming more important. Housekeeping genes that are absolutely reliable are essential for these studies to normalize gene expression. An i...

    Authors: Harun M Said, Carsten Hagemann, Jelena Stojic, Beate Schoemig, Giles H Vince, Michael Flentje, Klaus Roosen and Dirk Vordermark

    Citation: BMC Molecular Biology 2007 8:55

    Content type: Research article

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  34. Physiological long term adaptation of pancreatic beta cells is driven by stimuli such as glucose and incretin hormones acting via cAMP (e.g. GLP-1) and involves regulated gene expression. Several rapidly induc...

    Authors: Dominique A Glauser, Thierry Brun, Benoit R Gauthier and Werner Schlegel

    Citation: BMC Molecular Biology 2007 8:54

    Content type: Research article

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  35. Lipophorin receptors (LpRs) have been described in a number of insects, but functional studies have been reported only in locusts and mosquitoes. The aim of the present work was to characterize the LpR of the ...

    Authors: Laura Ciudad, Xavier Bellés and Maria-Dolors Piulachs

    Citation: BMC Molecular Biology 2007 8:53

    Content type: Research article

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  36. Zfra is a 31-amino-acid zinc finger-like protein, which is known to regulate cell death by tumor necrosis factor (TNF) and overexpressed TNF receptor- or Fas-associated death domain proteins (TRADD and FADD). ...

    Authors: Qunying Hong, Li-Jin Hsu, Lori Schultz, Nicole Pratt, Jeffrey Mattison and Nan-Shan Chang

    Citation: BMC Molecular Biology 2007 8:50

    Content type: Research article

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  37. Previous studies have demonstrated that telomeres in somatic cells are not randomly distributed at the end of the chromosomes. We hypothesize that these chromosome arm specific differences in telomere length (...

    Authors: Jesper Graakjaer, Rikke Christensen, Steen Kolvraa and Nedime Serakinci

    Citation: BMC Molecular Biology 2007 8:49

    Content type: Research article

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  38. Housekeeping genes are commonly used as endogenous reference genes for the relative quantification of target genes in gene expression studies. No conclusive systematic study comparing the suitability of differ...

    Authors: Monika Jung, Azizbek Ramankulov, Jan Roigas, Manfred Johannsen, Martin Ringsdorf, Glen Kristiansen and Klaus Jung

    Citation: BMC Molecular Biology 2007 8:47

    Content type: Research article

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  39. Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by ...

    Authors: Joseph D Szustakowski, Penelope A Kosinski, Christine A Marrese, Jee-Hyung Lee, Stephen J Elliman, Nanguneri Nirmala and Daniel M Kemp

    Citation: BMC Molecular Biology 2007 8:46

    Content type: Research article

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  40. The genetic code imposes a dilemma for cells. The DNA must be long enough to encode for the complexity of an organism, yet thin and flexible enough to fit within the cell. The combination of these properties g...

    Authors: Richard W Deibler, Jennifer K Mann, De Witt L Sumners and Lynn Zechiedrich

    Citation: BMC Molecular Biology 2007 8:44

    Content type: Research article

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  41. p63 is a transcription factor that plays an important role in skin epidermal development and differentiation. The p63 gene encodes for two major protein isoforms, those containing an amino-terminal trans-activ...

    Authors: Barbara Birkaya, Kori Ortt and Satrajit Sinha

    Citation: BMC Molecular Biology 2007 8:43

    Content type: Research article

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  42. Leptin acts not only on hypothalamic centers to control food intake but has additional functions in peripheral tissues, e.g. inhibition of insulin secretion from pancreatic islets. The leptin receptor (LEPRb) is ...

    Authors: Paul Hekerman, Julia Zeidler, Stefanie Korfmacher, Simone Bamberg-Lemper, Holger Knobelspies, Lennart Zabeau, Jan Tavernier and Walter Becker

    Citation: BMC Molecular Biology 2007 8:41

    Content type: Research article

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  43. Assessing RNA quality is essential for gene expression analysis, as the inclusion of degraded samples may influence the interpretation of expression levels in relation to biological and/or clinical parameters....

    Authors: Carina Strand, Johan Enell, Ingrid Hedenfalk and Mårten Fernö

    Citation: BMC Molecular Biology 2007 8:38

    Content type: Methodology article

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