Skip to main content

Advertisement

Articles

Page 13 of 17

  1. The major uptake system responsible for the transport of fructose, glucose, and sucrose in Corynebacterium glutamicum ATCC 13032 is the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The genes encodin...

    Authors: Lars Gaigalat, Jan-Philip Schlüter, Michelle Hartmann, Sascha Mormann, Andreas Tauch, Alfred Pühler and Jörn Kalinowski

    Citation: BMC Molecular Biology 2007 8:104

    Content type: Research article

    Published on:

  2. In situ detection of short sequence elements in genomic DNA requires short probes with high molecular resolution and powerful specific signal amplification. Padlock probes can differentiate single base variati...

    Authors: Jakob S Lohmann, Magnus Stougaard and Jørn Koch

    Citation: BMC Molecular Biology 2007 8:103

    Content type: Methodology article

    Published on:

  3. RNA Polymerase II (RNAP II) is recruited to core promoters by the pre-initiation complex (PIC) of general transcription factors. Within the PIC, transcription factor for RNA polymerase IIB (TFIIB) determines t...

    Authors: Gregory S Yochum, Veena Rajaraman, Ryan Cleland and Shannon McWeeney

    Citation: BMC Molecular Biology 2007 8:102

    Content type: Research article

    Published on:

  4. Varicella Zoster Virus Immediate Early 63 protein (IE63) has been shown to be essential for VZV replication, and critical for latency establishment. The activity of the protein as a transcriptional regulator i...

    Authors: Lionel Habran, Nadia El Mjiyad, Emmanuel Di Valentin, Catherine Sadzot-Delvaux, Sébastien Bontems and Jacques Piette

    Citation: BMC Molecular Biology 2007 8:99

    Content type: Research article

    Published on:

  5. Effective and stable knockdown of multiple gene targets by RNA interference is often necessary to overcome isoform redundancy, but it remains a technical challenge when working with intractable cell systems.

    Authors: Xiaocui Zhu, Leah A Santat, Mi Sook Chang, Jamie Liu, Joelle R Zavzavadjian, Estelle A Wall, Christine Kivork, Melvin I Simon and Iain DC Fraser

    Citation: BMC Molecular Biology 2007 8:98

    Content type: Methodology article

    Published on:

  6. Efficient and correct repair of DNA damage, especially DNA double-strand breaks, is critical for cellular survival. Defects in the DNA repair may lead to cell death or genomic instability and development of ca...

    Authors: Karin H Karlsson and Bo Stenerlöw

    Citation: BMC Molecular Biology 2007 8:97

    Content type: Research article

    Published on:

  7. Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays critical functions in many biological processes, including DNA repair and gene transcription. The main function of PARP-1 is to catalyze th...

    Authors: Karine Zaniolo, Serge Desnoyers, Steeve Leclerc and Sylvain L Guérin

    Citation: BMC Molecular Biology 2007 8:96

    Content type: Research article

    Published on:

  8. The prolactin-related proteins (PRPs) are non-classical placental-specific members of the prolactin/growth hormone family. Among ruminants, they are expressed in the cotyledonary villi of cattle and goat. We i...

    Authors: Koichi Ushizawa, Toru Takahashi, Misa Hosoe, Katsuhiro Ohkoshi and Kazuyoshi Hashizume

    Citation: BMC Molecular Biology 2007 8:95

    Content type: Research article

    Published on:

  9. In the C. albicans retrotransposon Tca2, the gag and pol ORFs are separated by a UGA stop codon, 3' of which is a potential RNA pseudoknot. It is unclear how the Tca2 gag UGA codon is bypassed to allow pol expres...

    Authors: Elaine M Forbes, Siân R Nieduszynska, Fiona K Brunton, Joanne Gibson, L Anne Glover and Ian Stansfield

    Citation: BMC Molecular Biology 2007 8:94

    Content type: Research article

    Published on:

  10. In functional genomics, transcript measurement is of fundamental importance. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays are the most popular technology and depend on the init...

    Authors: Jean-Philippe Levesque-Sergerie, Mathieu Duquette, Catherine Thibault, Louis Delbecchi and Nathalie Bissonnette

    Citation: BMC Molecular Biology 2007 8:93

    Content type: Methodology article

    Published on:

  11. The bacterial Sm-like protein Hfq is known as an important regulator involved in many reactions of RNA metabolism. A prominent function of Hfq is the stimulation of RNA polyadenylation catalyzed by E. coli poly(A...

    Authors: Marion Scheibe, Sonja Bonin, Eliane Hajnsdorf, Heike Betat and Mario Mörl

    Citation: BMC Molecular Biology 2007 8:92

    Content type: Research article

    Published on:

  12. Genomic DNA methylation affects approximately 1% of DNA bases in humans, with the most common event being the addition of a methyl group to the cytosine residue present in the CpG (cytosine-guanine) dinucleoti...

    Authors: Simon Hughes and J Louise Jones

    Citation: BMC Molecular Biology 2007 8:91

    Content type: Methodology article

    Published on:

  13. Human eosinophil-derived neurotoxin (edn) and eosinophil cationic protein (ecp) are members of a subfamily of primate ribonuclease (rnase) genes. Although they are generated by gene duplication event, distinct ed...

    Authors: Hsiu-Yu Wang, Hao-Teng Chang, Tun-Wen Pai, Chung-I Wu, Yuan-Hung Lee, Yen-Hsin Chang, Hsiu-Ling Tai, Chuan-Yi Tang, Wei-Yao Chou and Margaret Dah-Tsyr Chang

    Citation: BMC Molecular Biology 2007 8:89

    Content type: Research article

    Published on:

  14. Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active ...

    Authors: Hongshan Chen, Wei Mo, Huabo Su, Yanling Zhang and Houyan Song

    Citation: BMC Molecular Biology 2007 8:88

    Content type: Research article

    Published on:

  15. The 2,2,7-trimethylguanosine (TMG) cap structure is an important functional characteristic of ncRNAs with critical cellular roles, such as some snRNAs. Here we used immunoprecipitation with both K121 and R1131...

    Authors: Dong Jia, Lun Cai, Housheng He, Geir Skogerbø, Tiantian Li, Muhammad Nauman Aftab and Runsheng Chen

    Citation: BMC Molecular Biology 2007 8:86

    Content type: Research article

    Published on:

  16. Genetic experiments have clarified that p63 is a key transcription factor governing the establishment and maintenance of multilayered epithelia. Key to our understanding of p63 strategy is the identification o...

    Authors: Serena Borrelli, Barbara Testoni, Maurizio Callari, Daniela Alotto, Carlotta Castagnoli, Rose-Anne Romano, Satrajit Sinha, Alessandra M Viganò and Roberto Mantovani

    Citation: BMC Molecular Biology 2007 8:85

    Content type: Research article

    Published on:

  17. The homeodomain-containing transcription factor PITX3 was shown to be essential for normal eye development in vertebrates. Human patients with point mutations in PITX3 demonstrate congenital cataracts along with ...

    Authors: Satoru Sakazume, Elena Sorokina, Yoshiki Iwamoto and Elena V Semina

    Citation: BMC Molecular Biology 2007 8:84

    Content type: Research article

    Published on:

  18. Alternative splicing is a widespread mechanism of gene expression regulation. Previous analyses based on conventional RT-PCR reported the presence of an unspliced c-fos transcript in several mammalian systems. Co...

    Authors: Juan Jurado, Carlos A Fuentes-Almagro, María J Prieto-Álamo and Carmen Pueyo

    Citation: BMC Molecular Biology 2007 8:83

    Content type: Research article

    Published on:

  19. The Entamoeba histolytica EhrabB gene encodes for a Rab GTPase involved in phagocytosis. It is located at a virulence locus where the Ehcp112 gene is in the complementary strand at 332 bp of EhrabB start codon, s...

    Authors: Mónica Romero-Díaz, Consuelo Gómez, Israel López-Reyes, Máximo B Martínez, Esther Orozco and Mario A Rodríguez

    Citation: BMC Molecular Biology 2007 8:82

    Content type: Research article

    Published on:

  20. Genome integrity is constantly challenged and requires the coordinated recruitment of multiple enzyme activities to ensure efficient repair of DNA lesions. We investigated the dynamics of XRCC1 and PCNA that a...

    Authors: Oliver Mortusewicz and Heinrich Leonhardt

    Citation: BMC Molecular Biology 2007 8:81

    Content type: Research article

    Published on:

  21. The Gateway recombinatorial cloning system allows easy and rapid joining of DNA fragments. Here we report the construction and evaluation of three different Gram-positive vectors that can be used with the Mult...

    Authors: Tania M Perehinec, Saara NA Qazi, Sanyasi R Gaddipati, Vyvyan Salisbury, Catherine ED Rees and Philip J Hill

    Citation: BMC Molecular Biology 2007 8:80

    Content type: Methodology article

    Published on:

  22. MicroRNAs (miRNAs) are one of the most abundant groups of regulatory genes in multicellular organisms, playing important roles in many fundamental cellular processes. More than four hundred miRNAs have been id...

    Authors: Edyta Koscianska, Vesselin Baev, Konstantinia Skreka, Katerina Oikonomaki, Ventsislav Rusinov, Martin Tabler and Kriton Kalantidis

    Citation: BMC Molecular Biology 2007 8:79

    Content type: Methodology article

    Published on:

  23. We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inac...

    Authors: Raffaella Di Lisi, Anne Picard, Simonetta Ausoni and Stefano Schiaffino

    Citation: BMC Molecular Biology 2007 8:78

    Content type: Research article

    Published on:

  24. Most molecular biology experiments, and the techniques associated with this field of study, involve a great deal of engineering in the form of molecular cloning. Like all forms of engineering, perfect informat...

    Authors: Alexandre Angers-Loustau, Jeffrey Rainy and Kirmo Wartiovaara

    Citation: BMC Molecular Biology 2007 8:77

    Content type: Software

    Published on:

  25. Huntington's disease, spinal and bulbar muscular atrophy, and spinocerebellar ataxia 17 (SCA17) are caused by expansions in the polyglutamine (polyQ) repeats in Huntingtin protein (Htt), androgen receptor prot...

    Authors: Justin R Prigge and Edward E Schmidt

    Citation: BMC Molecular Biology 2007 8:76

    Content type: Research article

    Published on:

  26. The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the transformation of myelomonocytic cells by avian myeloblastosis virus (AMV). v-Myb is thought to exert its biologic...

    Authors: Holger Gundelach, Daniel Braas and Karl-Heinz Klempnauer

    Citation: BMC Molecular Biology 2007 8:75

    Content type: Research article

    Published on:

  27. Cone degeneration is the hallmark of the inherited retinal disease retinitis pigmentosa. We have previously identified a trophic factor "Rod-derived Cone Viability Factor (RdCVF) that is secreted by rods and p...

    Authors: Frédéric Chalmel, Thierry Léveillard, Céline Jaillard, Aurélie Lardenois, Naomi Berdugo, Emmanuelle Morel, Patrice Koehl, George Lambrou, Arne Holmgren, José A Sahel and Olivier Poch

    Citation: BMC Molecular Biology 2007 8:74

    Content type: Research article

    Published on:

  28. The tight organisation of eukaryotic genomes as chromatin hinders the interaction of many DNA-binding regulators. The local accessibility of DNA is regulated by many chromatin modifying enzymes, among them the...

    Authors: Roger Ferreira, Anton Eberharter, Tiziana Bonaldi, Mariacristina Chioda, Axel Imhof and Peter B Becker

    Citation: BMC Molecular Biology 2007 8:73

    Content type: Research article

    Published on:

  29. Tumors expressing a transforming growth factor-beta type I receptor (TβRI) mutant with sequence deletions in a nine-alanine (9A) stretch of the signal peptide are reported to be highly associated with disease ...

    Authors: Kuo-Li Chen, Wan-Hsin Liu, Yi-Yuan Yang, Sy-Jye C Leu and Neng-Yao Shih

    Citation: BMC Molecular Biology 2007 8:72

    Content type: Research article

    Published on:

  30. The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature termination codons (PTCs). In yeast Saccharomyces cerevisiae, the activity of the NMD pathway depends on...

    Authors: Svetlana Chabelskaya, Valentina Gryzina, Svetlana Moskalenko, Catherine Le Goff and Galina Zhouravleva

    Citation: BMC Molecular Biology 2007 8:71

    Content type: Research article

    Published on:

  31. Peroxisome proliferator-activated receptor delta (PPARδ) is a member of the nuclear receptor superfamily. Numerous studies have aimed at unravelling the physiological role of PPARδ as a transcriptional regulat...

    Authors: Kerstin Lundell, Petra Thulin, Anders Hamsten and Ewa Ehrenborg

    Citation: BMC Molecular Biology 2007 8:70

    Content type: Research article

    Published on:

  32. Enzymes involved in DNA metabolic events of the highly radioresistant bacterium Deinococcus radiodurans are currently examined to understand the mechanisms that protect and repair the Deinococcus radiodurans geno...

    Authors: Melanie Blasius, Rebecca Buob, Igor V Shevelev and Ulrich Hubscher

    Citation: BMC Molecular Biology 2007 8:69

    Content type: Research article

    Published on:

  33. Recent analysis of immune-related genes within the sea urchin genome revealed a number of large gene families with vertebrate homologues, such as the Toll-like and NOD/NALP-like receptor families and C-type le...

    Authors: Katherine M Buckley and L Courtney Smith

    Citation: BMC Molecular Biology 2007 8:68

    Content type: Research article

    Published on:

  34. Real-time quantitative PCR (qPCR) is a method for rapid and reliable quantification of mRNA transcription. Internal standards such as reference genes are used to normalise mRNA levels between different samples...

    Authors: Ann-Britt Nygard, Claus B Jørgensen, Susanna Cirera and Merete Fredholm

    Citation: BMC Molecular Biology 2007 8:67

    Content type: Methodology article

    Published on:

  35. Nucleolin is a major component of the nucleolus, but is also found in other cell compartments. This protein is involved in various aspects of ribosome biogenesis from transcription regulation to the assembly o...

    Authors: Iva Ugrinova, Karine Monier, Corinne Ivaldi, Marc Thiry, Sébastien Storck, Fabien Mongelard and Philippe Bouvet

    Citation: BMC Molecular Biology 2007 8:66

    Content type: Research article

    Published on:

  36. Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following t...

    Authors: Scott A Boden, Nadim Shadiac, Elise J Tucker, Peter Langridge and Jason A Able

    Citation: BMC Molecular Biology 2007 8:65

    Content type: Research article

    Published on:

  37. Cytokines have essential roles on intercellular communications and are effective in using a variety of intracellular pathways. Among this multitude of signalling pathways, the NF-κB (nuclear factor kappaB) and...

    Authors: Sandrine Lafarge, Hind Hamzeh-Cognasse, Patricia Chavarin, Christian Genin, Olivier Garraud and Fabrice Cognasse

    Citation: BMC Molecular Biology 2007 8:64

    Content type: Research article

    Published on:

  38. RNA interference (RNAi) is a regulatory mechanism conserved in higher eukaryotes. The RNAi pathway generates small interfering RNA (siRNA) or micro RNA (miRNA) from either long double stranded stretches of RNA...

    Authors: Zachary Klase, Prachee Kale, Rafael Winograd, Madhur V Gupta, Mohammad Heydarian, Reem Berro, Timothy McCaffrey and Fatah Kashanchi

    Citation: BMC Molecular Biology 2007 8:63

    Content type: Research article

    Published on:

  39. Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RT-qPCR) is the most accurate measure of gene expression in biological systems. The comparison of different samples requires th...

    Authors: Lindsey J Maccoux, Dylan N Clements, Fiona Salway and Philip JR Day

    Citation: BMC Molecular Biology 2007 8:62

    Content type: Research article

    Published on:

  40. Trinucleotide instability is a hallmark of degenerative neurological diseases like Huntington's disease, some forms of spinocerebellar ataxia and myotonic dystrophy type 1 (DM1). To investigate the effect of c...

    Authors: Walther JAA van den Broek, Derick G Wansink and Bé Wieringa

    Citation: BMC Molecular Biology 2007 8:61

    Content type: Research article

    Published on:

  41. CD36 is a multiligand receptor involved in various metabolic pathways, including cellular uptake of long-chain fatty acids. Defect function or expression of CD36 can result in dyslipidemia or insulin resistanc...

    Authors: Louisa Cheung, Malin Andersen, Carolina Gustavsson, Jacob Odeberg, Leandro Fernández-Pérez, Gunnar Norstedt and Petra Tollet-Egnell

    Citation: BMC Molecular Biology 2007 8:60

    Content type: Research article

    Published on:

  42. Japanese encephalitis virus (JEV) NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains. It is a key component of the viral RNA replicase comp...

    Authors: Yeon-Gu Kim, Ji-Seung Yoo, Jung-Hee Kim, Chan-Mi Kim and Jong-Won Oh

    Citation: BMC Molecular Biology 2007 8:59

    Content type: Research article

    Published on:

  43. Antisense oligonucleotides (AOs) can interfere with exon recognition and intron removal during pre-mRNA processing, and induce excision of a targeted exon from the mature gene transcript. AOs have been used in vi...

    Authors: Abbie M Adams, Penny L Harding, Patrick L Iversen, Catherine Coleman, Sue Fletcher and Steve D Wilton

    Citation: BMC Molecular Biology 2007 8:57

    Content type: Research article

    Published on:

  44. Gene transfer by electroporation (DNA electrotransfer) to muscle results in high level long term transgenic expression, showing great promise for treatment of e.g. protein deficiency syndromes. However little ...

    Authors: Pernille Hojman, John R Zibert, Hanne Gissel, Jens Eriksen and Julie Gehl

    Citation: BMC Molecular Biology 2007 8:56

    Content type: Research article

    Published on:

  45. Gene expression studies related to cancer diagnosis and treatment are becoming more important. Housekeeping genes that are absolutely reliable are essential for these studies to normalize gene expression. An i...

    Authors: Harun M Said, Carsten Hagemann, Jelena Stojic, Beate Schoemig, Giles H Vince, Michael Flentje, Klaus Roosen and Dirk Vordermark

    Citation: BMC Molecular Biology 2007 8:55

    Content type: Research article

    Published on: