Fig. 7From: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolismPSMD1 and PSMD2 localize to the surface of lipid droplets. Fluorescence expression vectors were constructed, including PSMD1-mCherry, PSMD2-mCherry, PSMD1-EGFP, PLIN2-EGFP, and Livedrop-EGFP. a PSMD1-mCherry or PSMD2-mCherry was transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). b PSMD1-EGFP and PSMD2-mCherry were co-transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). The fluorescence plot was analyzed by ImageJ software. c, d The cells were transfected with PSMD1-mCherry or PSMD2-mCherry for 48 h, and then the cells were treated with 200 μM oleic acid (OA) for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). e, f The cells were co-transfected with PSMD1-mCherry or PSMD2-mCherry and PLIN2-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). g, h The cells were co-transfected with PSMD1-mCherry or PSMD2-mCherry and Livedrop-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm)Back to article page