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Fig. 7 | BMC Molecular Biology

Fig. 7

From: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

Fig. 7

PSMD1 and PSMD2 localize to the surface of lipid droplets. Fluorescence expression vectors were constructed, including PSMD1-mCherry, PSMD2-mCherry, PSMD1-EGFP, PLIN2-EGFP, and Livedrop-EGFP. a PSMD1-mCherry or PSMD2-mCherry was transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). b PSMD1-EGFP and PSMD2-mCherry were co-transfected into cells for 48 h. Then, the cells were fixed and stained by DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). The fluorescence plot was analyzed by ImageJ software. c, d The cells were transfected with PSMD1-mCherry or PSMD2-mCherry for 48 h, and then the cells were treated with 200 μM oleic acid (OA) for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm). e, f The cells were co-transfected with PSMD1-mCherry or PSMD2-mCherry and PLIN2-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by laser scanning confocal microscope (bar = 10 μm). g, h The cells were co-transfected with PSMD1-mCherry or PSMD2-mCherry and Livedrop-EGFP vectors for 48 h, and then the cells were treated with 200 μM OA for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI. The images were captured by a laser scanning confocal microscope (bar = 10 μm)

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