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Fig. 5 | BMC Molecular Biology

Fig. 5

From: PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

Fig. 5

PSMD1 and PSMD2 regulate the expression level of fatty acids (FAs) and lipid synthesis-related genes. a Interference efficiency detection by qRT-PCR. b The expression level of fatty acid synthesis-related genes was detected by qRT-PCR. c The expression level of lipid synthesis-related genes was detected by qRT-PCR. d Overexpression efficiency detection by qRT-PCR. e The expression level of fatty acid synthesis-related genes was detected by qRT-PCR. f The expression level of lipid synthesis-related genes was detected by qRT-PCR. g The ASK1-p38-JNK and AKT signaling in groups of interfered cells and control cells was detected by Western Blot experiments. TUBULIN and GAPDH were the reference proteins. h Grey value analysis of g. ImageJ software was used for this analysis, according to the instructions. i The ASK1-p38-JNK and AKT signaling in the overexpression cell group and control cells was detected by Western Blot experiments. TUBULIN and GAPDH were the reference proteins. j Grey value analysis of i. ImageJ software was used for this analysis, according to the instructions. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 3; *p < 0.05; **p < 0.01) vs. NC

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