Table 1 Oligonucleotide sequences used for Taqman PCR and siRNA sequences
From: The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
| Primer name | Application | Sequence or assay ID |
|---|---|---|
| 1. Human BACE1-AS–F | Real-time PCR | GAAGGGTCTAAGTGCAGACATCTT |
| 2. Human BACE1-AS–R | Real-time PCR | AGGGAGGCGGTGAGAGT |
| 3. Human BACE1-AS–P | Real-time PCR | ACATTCTTCAGCAACAGCC |
| 4. Mouse BACE1-AS–F | Real-time PCR | GTAGGCAGGGAAGCTAGTACTGA |
| 5. Mouse BACE1-AS–R | Real-time PCR | AGAGGCTTGCAGTCCAGTTC |
| 6. Mouse BACE1-AS–P | Real-time PCR | CCTGGAAGGAGAAACAG |
| 7. Human BACE1–F | Real-time PCR | Taqman(Assay ID: Hs00201573_m1) |
| 8. Human BACE1–R | Real-time PCR | Taqman(Assay ID: Hs00201573_m1) |
| 9. Human BACE1–P | Real-time PCR | Taqman(Assay ID: Hs00201573_m1) |
| 10. Mouse BACE1–F | Real-time PCR | Taqman(Assay ID: Mm00478664_m1) |
| 11. Mouse BACE1–R | Real-time PCR | Taqman(Assay ID: Mm00478664_m1) |
| 12. Mouse BACE1–P | Real-time PCR | Taqman(Assay ID: Mm00478664_m1) |
| 13. Human BACE1-AS siRNA_a | siRNA | CCCTCTGACACTGTACCATCTCTTT |
| 14. Human BACE1-AS siRNA_b | siRNA | AGAAGGGTCTAAGTGCAGACATCTG |
| 15. Human BACE1-AS siRNA_c | siRNA | CCAGAAGAGAAAGGGCACT |