Fig. 2From: Comparison of miRNA-101a-3p and miRNA-144a-3p regulation with the key genes of alpaca melanocyte pigmentationEffect of miR-101a-3p and miR-144a-3p on microphthalmia-associated transcription factor (MITF) expression. HEK 293T cells were co-transfected with luciferase reporter plasmids carrying MITF 3′UTR sequences that were wild type (WT-MITF), mutated at miR-148a-3p binding site (MUT-MITF), or empty vector (GP-MITF). The luciferase activity of each sample was normalized to Renilla luciferase activity. Bars in each panel represent the mean ± standard error. **P < 0.01; ***P < 0.001Back to article page