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Fig. 2 | BMC Molecular Biology

Fig. 2

From: Comparison of miRNA-101a-3p and miRNA-144a-3p regulation with the key genes of alpaca melanocyte pigmentation

Fig. 2

Effect of miR-101a-3p and miR-144a-3p on microphthalmia-associated transcription factor (MITF) expression. HEK 293T cells were co-transfected with luciferase reporter plasmids carrying MITF 3′UTR sequences that were wild type (WT-MITF), mutated at miR-148a-3p binding site (MUT-MITF), or empty vector (GP-MITF). The luciferase activity of each sample was normalized to Renilla luciferase activity. Bars in each panel represent the mean ± standard error. **P < 0.01; ***P < 0.001

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