Fig. 4

DUSP1 was a direct target of miR-32-5p. Human CFs were transfected with a miR-32-5p inhibitor or mimic for 48 h. a A schematic representation of the DUSP1 3′UTR showing putative miRNA target sites is shown. b The analysis of the relative luminescence activities of DUSP1-WT and DUSP1-MT in human CFs is shown. The luminescence activity of DUSP-1 WT + mimics was relative to that of the DUSP1-WT NC group, and the luminescence activity of DUSP-1 MT + mimics was relative to that of the DUSP1-MT NC group. c The mRNA level of DUSP1 expression was quantified by a qRT-PCR analysis in the CFs of each experimental group. d The protein levels of DUSP1 were determined by a western blot assay in the CFs of each experimental group. β-actin was used as a loading control in western blot. The data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01 (compared with the NG group or the NC group), ^#p < 0.05, ^##p < 0.01 (compared with the HG Control group)