Fig. 2
Downregulated miR-32-5p in human CFs. a The expression level of miR-32-5p in the normal glucose (5.6 mM), osmotic control (5.6 mM glucose and 19.4 mM mannitol), and high glucose groups (25 mM). The mimic group was treated with miR-32-5p mimics for 48 h, and the inhibitor group was treated with a miR-32-5p inhibitor for 48 h. b The OD595 values of human CFs cultures in different experimental groups were determined by an MTT assay. c, d The results of an apoptosis assay in different experimental groups are shown. e, f The α-SMA expression of CFs, which was detected by a double-label immunofluorescence-staining technique, is shown red fluorescent cells indicate that α-SMA was expressed in CFs, Scale bar = 50 μm. The data are presented as the mean ± SEM (n = 3). *p < 0.05, **p < 0.01 (compared with the NG group), #p < 0.05, ##p < 0.01 (compared with the HG Control group)