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Fig. 4 | BMC Molecular Biology

Fig. 4

From: Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute

Fig. 4

Determination of the optimal number of internal genes for accurate normalization. The pairwise variation (V = Vn/Vn+1) was calculated between normalization factor NFn and NFn+1 was performed by GeNorm to determine the optimal number of reference genes required for qRT-PCR normalization in all the six sample sets. Values under 0.15 indicate that no additional genes are required for the normalization

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