Fig. 2

Knocking down of Sf-15 inhibited the proliferation and induced apoptosis and death of Sf9 cells. a Relative expression of Sf-15 after ASO transfection at different time-points. Mock means Sf9 cells transfected with antisense oligonucleotide of negative control (NC), ASO menas cells transfected with antisense oligonucleotides of Sf-15. U6 was used as an internal control. b DAPI staining showed chromatin condensation and apoptotic bodies in Sf9 cells after 72 h transfection of Sf-15 antisense oligonucleotide. Sf9 represented normal Sf9 cells, Mock and ASO refers to cells transfected with ASO of NC and Sf-15, respectively. Red arrows showed the apoptotic bodies. Scale bar represented 25 μm. c Apoptosis rate of cells that were transfected with antisense oligonucleotide of NC (Mock) and Sf-15 (ASO), respectively. d Apoptotic rates determined by Annexin-V/PI stain after transfection with antisense oligonucleotide of NC (Mock) and Sf-15 (ASO), respectively. The B3, B2, B4 and B1 quadrants in each panel represent the populations of normal, early and late apoptotic, and apoptotic necrotic cells, respectively. e Relative expression of Sf-15 after transfection of pBac[A3-EGFP-A3-Sf-15] (pBac-Sf-15) vector at different time-points. U6 was used as an internal control. f Growth curves of Sf9 cells detected by WST-8 method upon Sf-15 overexpression and repression. 6, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120 and 126 h represented different timepoint after transfection of Sf-15 antisense oligonucleotides (ASO) and pBac[A3-EGFP-A3-Sf-15] (pBac-Sf-15) respectively. Sf9 means normal Sf9 cells