Plasmid | Modified strain | Locus | Upstream region of homology | Downstream region of homology | Region deleted on chromosome/plasmid | Element(s) integrated onto chromosome/plasmid |
---|---|---|---|---|---|---|
pNZKOplaA::ErmFfluc | L. plantarum 423 plaA::erm-ffluc | plaA | 902Â bp upstream of plaA | 69Â bp internal fragment of plaA, 330Â bp plaB bacteriocin immunity gene and 214Â bp of plaC bacteriocin translocation protein | 208Â bp, including 102Â bp of plaA ORF | 3204Â bp erm-ffluc gene cassette |
pNZKOmunA::CatFfluc | E. mundtii ST4SA munA::cat-ffluc | munA | 911Â bp upstream of munA, including 135Â bp internal fragment of munA ORF | 633Â bp internal fragment of munB translocation gene | 260Â bp, including 18Â bp of munA ORF | 2471Â bp cat-ffluc gene cassette |
pNZKIaap::FRTerm | L. plantarum 423 aap::frt-erm | aap | 909Â bp internal fragment of aap ORF | 929Â bp internal fragment of aap ORF | None | 1458Â bp FRT-flanked erm gene |
pNZKIaap::FRTerm | L. plantarum 423 aap::frt_um | Â | 909Â bp internal fragment of aap ORF | 929Â bp internal fragment of aap ORF | 1063Â bp FRT-flanked erm resistance gene excised | 335Â bp including a 96Â bp FRT-scar |
pNZKOsrtA::FRTerm | E. mundtii ST4SA srtA::frt-erm | srtA | 1011Â bp upstream of srtA, including 57Â bp internal fragment of srtA ORF, 333Â bp mazG nucleotide pyrophosphohydrolase gene and 270Â bp fragment of mfs transporter ORF | 1011Â bp downstream of srtA, including 57Â bp internal fragment of srtA ORF and 576Â bp internal fragment of bglG transcriptional anti-terminator ORF | 580Â bp internal fragment of the srtA ORF | 1458Â bp FRT-flanked erm gene |
pNZKOsrtC::FRTerm | E. mundtii ST4SA srtC::frt-erm | srtC | 1011Â bp upstream of srtC, including 59Â bp of srtC ORF and 892Â bp of EbpCfm pilus subunit protein gene | 376Â bp downstream of srtA, including 60Â bp of srtC ORF and 225Â bp internal fragment of hemolysin III ORF | 726Â bp internal fragment of the srtC ORF | 1458Â bp FRT-flanked erm gene |