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Fig. 1 | BMC Molecular Biology

Fig. 1

From: Development of a novel selection/counter-selection system for chromosomal gene integrations and deletions in lactic acid bacteria

Fig. 1

Schematic representing the pNZmazFnisRK inducible mazF toxin vector used for the construction of double-crossover homologously recombined DNA integration or deletion mutants at any genomic loci. Relevant features are indicated, including restriction sites used for cloning; the E. coli/LAB repA and repC replication genes; the chloramphenicol acetyltransferase (cat) gene conferring resistance to chloramphenicol; the nisR and nisK nisin regulatory genes; and the nisin-inducible PnisA promoter from Lc. lactis pNZ9000. Integration cassettes are inserted via blunting into the BglII, HindIII or StuI restriction sites

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