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Table 2 Expression stability of the nine candidate reference genes as calculated by NormFinder

From: Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune

Rank ABA treatment MeJA treatment N treatment Different tissues All samples
Gene Stability Gene Stability Gene Stability Gene Stability Gene Stability
1 ACT 0.116 MUB 0.144 TIP41 0.049 EF1-α 0.041 MUB 0.188
2 EF1-α 0.184 α-TUB 0.153 EF1-α 0.075 CYP 0.041 EF1-α 0.191
3 MUB 0.247 EF1-α 0.271 CYP 0.098 α-TUB 0.084 ACT 0.327
4 UBC 0.275 TIP41 0.382 MUB 0.123 ACT 0.127 CYP 0.366
5 CYP 0.510 β-TUB 0.409 ACT 0.128 MUB 0.134 α-TUB 0.433
6 RPL 0.551 RPL 0.511 β-TUB 0.279 RPL 0.232 RPL 0.440
7 α-TUB 0.592 CYP 0.574 UBC 0.322 β-TUB 0.662 β-TUB 0.541
8 TIP41 0.633 ACT 0.614 RPL 0.364 UBC 0.753 TIP41 0.694
9 β-TUB 0.782 UBC 1.683 α-TUB 0.464 TIP41 1.172 UBC 0.942