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Table 1 Candidate reference genes, primer sequences, and characteristics of PCR amplification in Isatis indigotica

From: Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune

Gene symbol Gene ID Gene name Primer: forward/reverse Arabidopsis ortholog no. Identify (%) Amplicon size (bp) E (%) R 2
ACT GARR01013235.1 Actin GCTCACGGAAGCACCT
CGACCACTAGCGTAAAGT
At3g53750 95 128 91 0.9984
UBC GARR01010405.1 Ubiquitin-conjugating enzyme TTTGCTGGAAAGGGAC
TTGGAGGTTTGAAAGGAT
At1g50490 92 105 107 0.9944
α-TUB GARR01008456.1 Alpha-tubulin GAGCCTTTGTTCATTG
CAACCTCCTCATAATCC
At1g50010 83 102 109 0.9968
β- TUB GARR01020827.1 Beta-tubulin TAAAGAAGTGGACGAAC
CCCTTAGGAGGAATGT
At5g12250 88 111 96 0.9987
EF1-α GARR01019351.1 Elongation factor 1-α GCCGATTGTGCTGTCC
GTGGCATCCATCTTGTTA
At1g07930 96 149 94 0.9859
MUB GARR01001157.1 Membrane-anchored ubiquitin-fold protein TTTCCCGATGCTACAA
ACCTGAGGCTGAATGA
At5g15460 90 215 109 0.9969
CYP GARR01006610.1 Cyclophilin GGCAAGACAGTTCCTA
TGATTCTCCACCCATA
At2g29960 89 177 99 0.9975
RPL GARR01002832.1 Ribosomal protein L18 CAAGGGCTAGGATTGA
TAAGGTTTGGAGTGGC
At5g27850 90 172 106 0.9976
TIP41 GARR01011419.1 TIP41-like family protein AACACTTGAGCAGCAA
GTATGGCGTCGTAAAA
At4g34270 93 173 105 0.9980