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Fig. 3 | BMC Molecular Biology

Fig. 3

From: MEF2A alters the proliferation, inflammation-related gene expression profiles and its silencing induces cellular senescence in human coronary endothelial cells

Fig. 3

DEGs between MEF2A silencing group and NC-group. a The heatmap of the DEGs. Three groups of HCAEC transfected with MEF2A-1527 were respectively indicated with 1527-1, 1527-2 and 1527-3. The mixed sample with three groups of HCAEC transfected with negative control siRNA was defined as NC. b, c The selected DEGs were validated with real-time quantitative PCR. The relative expression of each gene was calculated according to 2−((Cte1−Cte0) −(Ctn1−Ctn0)), here, Cte1 and Cte0 respectively indicate the Ct value of the target gene and the internal reference gene (beta-actin) in MEF2A siRNA-groups; Ctn1 and Ctn0 respectively indicate the Ct value of the target gene and beta-actin in the NC groups. The relative expression of all selected genes was normalized to “1” in the NC groups. c These genes have at least one potential MEF2A binding site in its proximal promoter. *p < 0.05, **p < 0.01, ***p < 0.001 are significant

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