Transcriptomic responses to grazing reveal the metabolic pathway leading to the biosynthesis of domoic acid and highlight different defense strategies in diatoms

Table 6 Genes regulated in the domoic acid (DA) induced grazer treatments that are involved or suggested to be involved in the biosynthesis of DA

Domoic acid
Accession number	Mean FC	Adjusted P value	Gene name	Description	Enzyme EC:
In the methylerythritol phosphate metabolic pathway (MEP) to geranyl pyrophosphate (GPP)
PSN0016235	1.88	< 0.001	Dxr	1-Deoxy-d-xylulose 5-phosphate reductoisomerase	1.1.1.267
PSN0004050	1.82	< 0.001	ispH	4-Hydroxy-3-methylbut-2-enyl diphosphate reductase	1.17.1.2
PSN0005356	1.61	0.043	ispG	4-Hydroxy-3-methylbut-2-en-1-yl diphosphate synthase	1.17.7.1
PSN0002600	1.60	< 0.001	ispD	2-C-Methyl-d-erythritol 4-phosphate cytidylyltransferase, chloroplastic	2.7.7.60
PSN0023754	1.60	0.013	ispE	4-Diphosphocytidyl-2-C-methyl-d-erythritol kinase	2.7.1.148
In the synthesis from MEP to GPP and l-glutamate towards domoic acid
PSN0015669	12.23	< 0.001	DabB	Hypothetical protein	NA
PSN0029114 + PSN002498	11.23	< 0.001	DabC	Dioxygenase	NA
PSN0007588	9.41	< 0.001	DabA	N-prenyltransferase	NA
PSN0005772	2.83	<0.001	idi1-2	Isopentenyl-diphosphate Delta-isomerase	5.3.3.2
PSN0020364	2.83	< 0.001	DabD	Cytochrome P450	NA
Proline metabolism
PSN0000830	1.67	< 0.001	P4HA	Prolyl 4-hydroxylase	1.14.11.2
PSN0001687	1.62	0.002	NA	Ornithine cyclodeaminase	4.3.1.12