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Fig. 2 | BMC Molecular Biology

Fig. 2

From: A fragment activity assay reveals the key residues of TBC1D15 GTPase-activating protein (GAP) in Chiloscyllium plagiosum

Fig. 2

Protein expression and purification. Proteins were analyzed by SDS-PAGE. Lane 1, 4, 7, 10 displays the proteins with a His-sumo tag. Lane 2, 5, 8, 11 shows the proteins that were cut by the Ulp1 enzyme. Lane 3, 6, 9, 12 displays the purified proteins. a Shark-TBC1D15-GAP/mutation, Sus-TBC1D15-GAP and Homo-TBC1D15-GAP-mutation. b Sus-TBC1D15-GAP-mutation and Homo-TBC1D15-GAP. c Rab4/5/7/11

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