Skip to main content

Advertisement

Table 3 Breakpoints identified within the AF9 SAR region in H2O2-treated cells

From: Matrix association region/scaffold attachment region (MAR/SAR) sequence: its vital role in mediating chromosome breakages in nasopharyngeal epithelial cells via oxidative stress-induced apoptosis

Cell line treated with H2O2 Breakpoint Remarks
NP69 245,566 This chromosome break was detected within the AF9 region (at coordinates 245,252–245,612) which was previously found to take part in the MLL-AF9 translocation in an ALL patient [GenBank:AM050804]. This breakpoint is 46 nucleotides different from that identified in an ALL patient (at coordinate 245,612) [GenBank:AM050804]
245,591 This chromosome break was detected within the AF9 region (at coordinates 245,252–245,612) which was previously found to take part in the MLL-AF9 translocation in an ALL patient [GenBank:AM050804]. This breakpoint is two nucleotides different from that reported in cultured normal blood cells treated with VP16 (at coordinate 245,593) [35] and 21 nucleotides different from that identified in an ALL patient (at coordinate 245,612) [GenBank:AM050804]
245,645 This breakpoint is 33 nucleotides different from that identified in an ALL patient (at coordinate 245,612) [GenBank:AM050804]
245,659 This breakpoint is 47 nucleotides different from that identified in an ALL patient (at coordinate 245,612) [GenBank:AM050804]
245,711  
245,730  
245,804  
245,817  
245,826  
245,842  
245,959  
245,970  
246,089  
HK1 245,560 This chromosome break was detected within the AF9 region (at coordinates 245,252–245,612) which was previously found to take part in the MLL-AF9 translocation in an ALL patient [GenBank:AM050804]. This breakpoint is 52 nucleotides different from that identified in an ALL patient (at coordinate 245,612) [GenBank:AM050804]
245,634 This breakpoint is 22 nucleotides different from that identified in an ALL patient (at coordinate 245,612) [GenBank:AM050804]
245,681 This breakpoint is 69 nucleotides different from that identified in an ALL patient (at coordinate 245,612) [GenBank:AM050804]
245,755  
245,949  
  1. The nucleotide positions of the chromosome breaks identified within the AF9 SAR region were mapped according to the AF9 sequence retrieved from Ensembl database [EMBL:ENSG00000171843]