Fig. 5
Caspase-3 inhibitor abolishes H2O2-induced cleavages within the AF9 SAR region. a Representative gel pictures showing the IPCR analysis of the AF9 SAR region in H2O2-treated HK1 cells: i without CI pre-treatment ii with CI pre-treatment. HK1 cells were left untreated or pre-treated with 50 µM of Z-DEVD-FMK for 1 h. The cells were then either untreated (lanes 2–7) or treated with 50 µM of H2O2 for 8 h (lanes 8–13). Genomic DNA was extracted and modified for nested IPCR. For each cell sample, six IPCR replicates (R1–R6) were prepared. The side brackets show the IPCR bands derived from the cleavages of the AF9 gene. M: 100 bp DNA ladder. N: Negative control for IPCR. b Cleavage frequency of the AF9 SAR region detected in HK1 cells. Data are representative of two independent experiments. Each experiment consisted of 1–2 sets of IPCR. Each set of IPCR was performed in 5–6 IPCR replicates per cell sample. The results are expressed as medians with IQRs. *P < 0.05 (Mann–Whitney U test)