Fig. 2

Cleavage frequencies of the AF9 SAR and non-SAR regions in H₂O₂-treated NP69 cells. a Representative gel pictures showing the IPCR results of the AF9 gene in H₂O₂-treated NP69 cells: i SAR region, ii Non-SAR region. NP69 cells were either untreated (lanes 2–7) or treated with 100 µM of H₂O₂ for 16 h (lanes 8–13). The cells were harvested for gDNA extraction and nested IPCR. For each cell sample, six IPCR replicates (R1–R6) were prepared. The side brackets show the IPCR bands derived from the cleavages of the AF9 gene. M: 100 bp DNA ladder. N: Negative control for IPCR. b AF9 cleavage frequency detected in NP69 cells. Data are representative of three independent experiments. Each experiment consisted of 1–2 sets of IPCR. Each set of IPCR was performed in 5–8 IPCR replicates per cell sample. The results are presented as medians with IQRs. *P < 0.05; NS: no significant difference (Mann–Whitney U test)