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Fig. 5 | BMC Molecular Biology

Fig. 5

From: The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replication

Fig. 5

Nucleotide hydrolysis by purified Twm1 in vitro. a Hydrolysis of both rNTPs (grey bars) and dNTPs (white bars) by Twm1 was performed at 21 °C. b NTPase activity of Twm1 was also measured in the presence of linear dsDNA (FHA0; black bars), circular ssDNA (M13mp18; grey bars) or linear ssDNA (FHA3.1; hatched bars) at 21 °C. Error bars represent the standard error. p values were calculated using Student’s t-test (*< 0.05; **< 0.005; ***< 0.001). All values were normalized against no protein negative controls (empty vector purification)

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