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Fig. 3 | BMC Molecular Biology

Fig. 3

From: The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replication

Fig. 3

Relative twm1 mRNA levels of D. discoideum AX2 in response to EtBr treatment. Initial AX2 cultures (T0) were first treated with 10 μg/ml EtBr for 24 h (T24), at which point the EtBr-containing medium was removed and replaced. Following this, cells were allowed a further 24 h to recover without EtBr (T48). A duplicate culture without EtBr treatment was used as a control. twm1 mRNA levels were quantified with qRT-PCR and normalized against a structural gene (tubB). Values are relative to initial (T0) mRNA level. Error bars represent the standard error. p values were calculated using Student’s t-test (*< 0.05; **< 0.0001)

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