TY - JOUR AU - Boggiatto, Paola M. AU - Fitzsimmons, Daniel AU - Bayles, Darrell O. AU - Alt, David AU - Vrentas, Catherine E. AU - Olsen, Steven C. PY - 2018 DA - 2018/08/01 TI - Coincidence cloning recovery of Brucella melitensis RNA from goat tissues: advancing the in vivo analysis of pathogen gene expression in brucellosis JO - BMC Molecular Biology SP - 10 VL - 19 IS - 1 AB - Brucella melitensis bacteria cause persistent, intracellular infections in small ruminants as well as in humans, leading to significant morbidity and economic loss worldwide. The majority of experiments on the transcriptional responses of Brucella to conditions inside the host have been performed following invasion of cultured mammalian cells, and do not address gene expression patterns during long-term infection. SN - 1471-2199 UR - https://doi.org/10.1186/s12867-018-0111-x DO - 10.1186/s12867-018-0111-x ID - Boggiatto2018 ER -