Fig. 4

PC4 affects the apparent 3′ end processing of RDH pre-mRNAs. RT-qPCR analyses of RDH transcripts (and two RIH transcripts as controls) were performed in PC4 OE (left panel) and PC4 KD (right panel) cells synchronized to S or G1 phase. The charts represent the ratio between “3′ end” region (which is located downstream of the cleavage site and corresponds to extended transcripts) and “TSS” region (which corresponds to total mRNA level). Error bars indicate standard deviations (SD) of three biological replicates. P-values were calculated on relative level of expression values using Student’s T-test and statistical significance is represented as follows: *P ≤ 0.05