Fig. 2

RNA polymerase II occupancy on histone genes in PC4 OE cells (left panel) and PC4 KD cells (right panel) synchronized to G1 and S phase. Analysis were done by ChIP-seq (for PC4 OE cells) and ChIP qPCR (for PC4 OE and PC4 KD cells). Charts represent mean fold change value (n = 3 for ChIP-qPCR and n = 1 for ChIP-seq). Regions are marked as described in Fig. 1c. As a negative control, two RIH genes (H2AFZ and H3F3A) as well as two intergenic regions were tested. Values were normalized to data obtained from control cells (marked by horizontal lines): EBFP OE for PC4 OE cells and HeLa scramble for PC4 KD cells. Error bars indicate standard deviations (SD) of three biological replicates. P-values were calculated on percent of input values using Student’s T-test and statistical significance is represented as follows: *P ≤ 0.05