Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions

Table 2 Stability ranking of seven candidate reference genes by geNorm, NormFinder, and BestKeeper

Group	Rank	geNorm		NormFinder		BestKeeper
Group	Rank	Gene	Stability	Gene	Stability	Gene	SD dev [± CP]
Different tissues and fruit developmental stages	1	DBP	0.96	GAPDH	0.291	ACTIN1	0.98
	2	HISTH4	0.96	HISTH4	0.352	HISTH4	1.41
	3	GAPDH	1.07	DBP	0.443	DBP	1.57
	4	18S	1.24	18S	0.699	GAPDH	1.94
	5	ACTIN1	1.54	ACTIN1	0.781	18S	2.22
	6	ACTIN2	1.87	ACTIN2	1.036	ACTIN2	2.62
	7	PIRUV	2.20	PIRUV	1.211	PIRUV	2.68
Light quality	1	GAPDH	0.72	GAPDH	0.251	HISTH4	1.05
	2	DBP	0.72	DBP	0.335	DBP	1.30
	3	ACTIN1	0.85	ACTIN1	0.347	ACTIN1	1.45
	4	HISTH4	1.00	HISTH4	0.810	GAPDH	1.67
	5	18S	1.24	18S	1.034	18S	1.81
	6	ACTIN2	1.51	ACTIN2	1.286	PIRUV	2.60
	7	PIRUV	1.75	PIRUV	1.501	ACTIN2	2.84
Low temperature	1	ACTIN1	0.56	HISTH4	0.190	ACTIN1	0.29
	2	HISTH4	0.56	ACTIN1	0.211	HISTH4	0.38
	3	PIRUV	0.67	PIRUV	0.438	PIRUV	0.38
	4	GAPDH	0.74	DBP	0.501	DBP	0.58
	5	DBP	0.78	GAPDH	0.544	GAPDH	0.58
	6	18S	0.99	18S	0.864	18S	0.82
	7	ACTIN2	1.18	ACTIN2	1.041	ACTIN2	1.17
Total	1	DBP	0.98	HISTH4	0.243	HISTH4	0.95
	2	HISTH4	0.98	GAPDH	0.307	DBP	1.25
	3	GAPDH	1.08	DBP	0.406	GAPDH	1.54
	4	ACTIN1	1.20	ACTIN1	0.472	18S	1.78
	5	18S	1.44	ACTIN2	0.592	PIRUV	2.74
	6	ACTIN2	1.67	18S	0.672	ACTIN2	2.91
	7	PIRUV	1.91	PIRUV	0.853	ACTIN1	3.18

ISSN: 1471-2199