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Table 2 Stability ranking of seven candidate reference genes by geNorm, NormFinder, and BestKeeper

From: Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions

Group Rank geNorm NormFinder BestKeeper
Gene Stability Gene Stability Gene SD dev [± CP]
Different tissues and fruit developmental stages 1 DBP 0.96 GAPDH 0.291 ACTIN1 0.98
2 HISTH4 0.96 HISTH4 0.352 HISTH4 1.41
3 GAPDH 1.07 DBP 0.443 DBP 1.57
4 18S 1.24 18S 0.699 GAPDH 1.94
5 ACTIN1 1.54 ACTIN1 0.781 18S 2.22
6 ACTIN2 1.87 ACTIN2 1.036 ACTIN2 2.62
7 PIRUV 2.20 PIRUV 1.211 PIRUV 2.68
Light quality 1 GAPDH 0.72 GAPDH 0.251 HISTH4 1.05
2 DBP 0.72 DBP 0.335 DBP 1.30
3 ACTIN1 0.85 ACTIN1 0.347 ACTIN1 1.45
4 HISTH4 1.00 HISTH4 0.810 GAPDH 1.67
5 18S 1.24 18S 1.034 18S 1.81
6 ACTIN2 1.51 ACTIN2 1.286 PIRUV 2.60
7 PIRUV 1.75 PIRUV 1.501 ACTIN2 2.84
Low temperature 1 ACTIN1 0.56 HISTH4 0.190 ACTIN1 0.29
2 HISTH4 0.56 ACTIN1 0.211 HISTH4 0.38
3 PIRUV 0.67 PIRUV 0.438 PIRUV 0.38
4 GAPDH 0.74 DBP 0.501 DBP 0.58
5 DBP 0.78 GAPDH 0.544 GAPDH 0.58
6 18S 0.99 18S 0.864 18S 0.82
7 ACTIN2 1.18 ACTIN2 1.041 ACTIN2 1.17
Total 1 DBP 0.98 HISTH4 0.243 HISTH4 0.95
2 HISTH4 0.98 GAPDH 0.307 DBP 1.25
3 GAPDH 1.08 DBP 0.406 GAPDH 1.54
4 ACTIN1 1.20 ACTIN1 0.472 18S 1.78
5 18S 1.44 ACTIN2 0.592 PIRUV 2.74
6 ACTIN2 1.67 18S 0.672 ACTIN2 2.91
7 PIRUV 1.91 PIRUV 0.853 ACTIN1 3.18