Gene name | Accession number | Primer sequence (5′-3′) forward/reverse | Amplicon size (bp) | Annealing Tm (°C) | Melting Tm (°C) | E (%) | R2 | References |
---|---|---|---|---|---|---|---|---|
ACTIN1 | LC017712.1 | TTCACGAGACCACCTATAACTC GCTCATCCTATCAGCGATT | 122 | 55 | 81 | 100.3 | 0.994 | – |
ACTIN2 | AB116565.1 | GCTAATCGTGAGAAGATGAC AGCACAATACCAGTAGTACG | 119 | 55 | 81.5 | 97.8 | 0.998 | [21] |
18S | X15590.1 | TGTGAAACTGCGAATGGCTCATTAA GAAGTCGGGATTTGTTGCACGTATT | 110 | 60 | 78.5 | 86 | 0.998 | [15] |
HISTH4 | AB197150.1 | TCAAGCGTATCTCCGGTCTC AGTGTCCTTCCCTGCCTCTT | 163 | 60 | 85.5 | 92.9 | 0.999 | [22] |
GAPDH | AB363963.1 | GAGTCTACTGGAGTGTTCA CTTGTATTCGTGCTCATTCA | 135 | 55 | 83.5 | 104.8 | 0.998 | [23] |
DBP | XM_004291635.2 | TTGGCAGCGGGACTTTACC CGGTTGTGTGACGCTGTCAT | 72 | 60 | 80 | 108.6 | 0.995 | [24] |
PIRUV | AF141016.2 | AGGTGCGTTGCGAAGAGGA CTAAATCTGTGAATGCGAATGAGG | 219 | 60 | 84.5 | 103.5 | 0.990 | [15] |