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Table 1 Description of seven candidate reference genes, primer sequences, and amplicon characteristics

From: Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions

Gene name

Accession number

Primer sequence (5′-3′) forward/reverse

Amplicon size (bp)

Annealing Tm (°C)

Melting Tm (°C)

E (%)

R2

References

ACTIN1

LC017712.1

TTCACGAGACCACCTATAACTC

GCTCATCCTATCAGCGATT

122

55

81

100.3

0.994

ACTIN2

AB116565.1

GCTAATCGTGAGAAGATGAC

AGCACAATACCAGTAGTACG

119

55

81.5

97.8

0.998

[21]

18S

X15590.1

TGTGAAACTGCGAATGGCTCATTAA

GAAGTCGGGATTTGTTGCACGTATT

110

60

78.5

86

0.998

[15]

HISTH4

AB197150.1

TCAAGCGTATCTCCGGTCTC

AGTGTCCTTCCCTGCCTCTT

163

60

85.5

92.9

0.999

[22]

GAPDH

AB363963.1

GAGTCTACTGGAGTGTTCA

CTTGTATTCGTGCTCATTCA

135

55

83.5

104.8

0.998

[23]

DBP

XM_004291635.2

TTGGCAGCGGGACTTTACC

CGGTTGTGTGACGCTGTCAT

72

60

80

108.6

0.995

[24]

PIRUV

AF141016.2

AGGTGCGTTGCGAAGAGGA

CTAAATCTGTGAATGCGAATGAGG

219

60

84.5

103.5

0.990

[15]