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Table 1 AMO designs used in this work and respective theoretical melting temperatures

From: Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides

Length (nt) Name Type Sequence (5′-3′) Predicted Tm (°C)
16 AM_ LNA_PS LNA/DNA GC+TAG+ATA+ACC+AAA+GA 57.92
16 AM_LNA_2OMe_PS 2′-OMe/LNA gc+Uag+Aua+Acc+Aaa+Ga 77.60
16 SC_LNA_PS LNA/DNA GC+TAG+ATA+AGC+TAA+GA 51.95
19 SC_LNA_PS* LNA/DNA TAA+CAC+GTCT+ATAC+GCC+CA 61.03
16 FAM_AM_ LNA_PS LNA/DNA FAM_GC+TAG+ATA+ACC+AAA+GA 57.92
16 FAM_SC_LNA_PS LNA/DNA FAM_GC+TAG+ATA+AGC+TAA+GA 51.95
  1. LNA nucleotide monomers are represented by the symbol “+” before the letter, 2′-OMe-RNA monomers in small caps letters, DNA nucleotides in capital letters, and FAM—fluorescein; the symbol asterisk refers to the scramble sequence available commercially