Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides

BMC Molecular Biology

Table 1 AMO designs used in this work and respective theoretical melting temperatures

Length (nt)	Name	Type	Sequence (5′-3′)	Predicted T_m (°C)
16	AM_ LNA_PS	LNA/DNA	GC+TAG+ATA+ACC+AAA+GA	57.92
16	AM_LNA_2OMe_PS	2′-OMe/LNA	gc+Uag+Aua+Acc+Aaa+Ga	77.60
16	SC_LNA_PS	LNA/DNA	GC+TAG+ATA+AGC+TAA+GA	51.95
19	SC_LNA_PS*	LNA/DNA	TAA+CAC+GTCT+ATAC+GCC+CA	61.03
16	FAM_AM_ LNA_PS	LNA/DNA	FAM_GC+TAG+ATA+ACC+AAA+GA	57.92
16	FAM_SC_LNA_PS	LNA/DNA	FAM_GC+TAG+ATA+AGC+TAA+GA	51.95

LNA nucleotide monomers are represented by the symbol “+” before the letter, 2′-OMe-RNA monomers in small caps letters, DNA nucleotides in capital letters, and FAM—fluorescein; the symbol asterisk refers to the scramble sequence available commercially

ISSN: 1471-2199