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Fig. 3 | BMC Molecular Biology

Fig. 3

From: Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides

Fig. 3

Cellular uptake assay of the labeled AMOs using MKN74 cells. a Representative epifluorescence microscopy images obtained with FAM_AM_LNA_PS at different concentrations (FAM_AM_LNA_PS/5 nM,/50 nM and/100 nM) in a hybridization solution containing 0.5 M of urea. The hybridization step was performed at 37 °C for 2 h, followed by 30 min of washing. As negative controls, cells without any sequence (control) or incubated with FAM_SC_LNA_PS were used. The FAM_SC_LNA_PS/0.5M_30 min, and FAM_SC_LNA_PS/0.5M_60 min corresponds to the FISH assay using the FAM_SC_LNA_PS hybridized for 2 h using a solution containing 0.5 M of urea, followed by a washing step of 30 and 60 min, respectively. All images were acquired using an ×200 magnification. b Average fluorescence intensity for the FAM_AM_LNA_PS at 5, 50, and 100 nM; and c for the FAM_SC_LNA_PS using different concentrations of urea in the hybridization solution and washing times. Fluorescent signal intensity is expressed in arbitrary fluorescent units (AFU); ns—not significantly different from control (p > 0.05). d Cellular uptake assay using the FAM_AM_LNA_PS at 100 nM (FAM_AM_LNA_PS/naked) applied directly into the medium after 2 and 48 h of hybridization. DAPI was used to stain the nucleus. The image was acquired using an ×400 magnification

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