Table 4 Primers utilized to detect and sequence FOXO3 gene disruption
| Primer name | Sequence |
|---|---|
| FOXO3 F (for detection of disruption) | 5′-GTGCTTCAGGATCGCTTCA-3′ |
| Neo cassette R (for detection of disruption) | 5′-TGCATGCTTTGCATACTTCTG-3′ |
| FOXO3 seq. (for sequencing disruption mutants) | 5′-CTCGGTTTTGGACCATTCTG-3′ |