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Table 3 CRISPR Cas9 mutation frequencies in mammalian cell lines

From: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone

Cell line G418-resistant isolates screened by western blot analysis Number of homozygous truncation mutants Homozygous mutation frequency (%)
U87MG Trial 1 77 4a 5
U87MG Trial 2 50 3a 6
BT549 56 5 9
HEK293 77 1 1.3
  1. aThese mutants are shown in western blot analyses found in Figs. 4, 5