Fig. 3From: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backboneSchematic of FOXO3 gene disruption with neomycin resistance cassette (NPTII). Guide RNAs were employed to nick FOXO3 gene in mammalian cell lines. The lesions were resolved by recombination with a donor vector that contained a neomycin resistance gene (NPTII) flanked by FOXO3 sequences (that were proximal to the CRISPR Cas9-generated nicked strands of FOXO3 in the chromosome)Back to article page