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Fig. 3 | BMC Molecular Biology

Fig. 3

From: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone

Fig. 3

Schematic of FOXO3 gene disruption with neomycin resistance cassette (NPTII). Guide RNAs were employed to nick FOXO3 gene in mammalian cell lines. The lesions were resolved by recombination with a donor vector that contained a neomycin resistance gene (NPTII) flanked by FOXO3 sequences (that were proximal to the CRISPR Cas9-generated nicked strands of FOXO3 in the chromosome)

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